CT26.WT cell CT26.WT 细胞 NICR1724 cell NICR1724 细胞 3131C0001000800037 Base Medium+8%DMSO+20%FBS CT26 is an undifferentiated colon cancer cell line induced by N-nitroso-N-methylurethane-(NNMU). Its cloned cell line was named as CT26.WT (ATCC CRL-2638). CT26.WT was stably transfected with a retroviral vector LXSN containing the lacZ gene encoding a typical tumor-associated antigen (TAA) beta-galactosidase (beta-gal), resulting in a lethal subclone CT26.CL25 (ATCC CRL-2639). Even if CT26.CL25 expressed a typical TAA beta-galactosidase, CT26.CL25 and CT2 in normal mice, the growth rate and lethality of CT26.CL25 and CT2.WT in normal mice are also consistent. The cells can be used as a model for testing immunotherapy programs and for the study of host immune responses together with CT26.CL25(ATCC CRL-2639). Cultures submitted to the ATCC in July 2001 were contaminated Mycoplasma. Mycoplasma was eliminated by its progeny by BM cyclin treatment for 21 days. Six weeks after the treatment, Mycoplasma was tested using Hoechst staining, PCR and standard culture tests. The results were negative. CT26.WT CT26.WT cell CT26WT-cell morphology-SHCRC Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Mouse Colon Cancer Cells; CT26.WT NICR1724 Negative PN5 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth fibroblasts http://www.cellresource.cn/fdetail.aspx?id=1724 http://www.cellresource.cn/pdf/20080108101040.pdf A类 CT26 是以N-nitroso-N-methylurethane-(NNMU)诱导形成的未分化结肠癌细胞株。 它克隆形成的细胞株命名为CT26.WT (ATCC CRL-2638)。 用包含编码典型肿瘤相关抗原(TAA) beta-半乳糖苷酶(beta-gal)的lacZ基因的逆转录病毒载体LXSN稳定转染CT26.WT，得到致死的亚克隆CT26.CL25 (ATCC CRL-2639)。 即使CT26.CL25表达了典型的TAA beta-半乳糖苷酶，在正常小鼠中CT26.CL25 和 CT2 CT26.WT 细胞 CT26WT-细胞形态-SHCRC PN5 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 小鼠结肠癌细胞；CT26.WT 成纤维细胞 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性