L-M(TK–) cell L-M(TK–) 细胞 NICR770 cell NICR770 细胞 3142C0001000000002 Base Medium+8%DMSO+20%FBS C36 Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose)  10%FBS Isoenzyme Identification L-M (TK-) cells are derivatives of the L-M (ATCC-CCL-1.2) cell line with the characteristic anti-5-bromodeoxynucleoside (5-bromo-2-deoxyridine), thymidine kinase deficiency (thymidine kinase mutant), and are widely used in fundamental research of genetics and biochemistry. It can also be used in the experimental studies of somatic hybridization and gene delivery. L-M(TK-) L-M(TK-) cell Mouse Connective Tissue Thymidine Kinase-deficient Strain; L-M(TK-) NICR770 Negative Passage at 1: 3, and passage once in 2-3 days. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=770 http://www.cellresource.cn/pdf/20080108100710.pdf 1:3传代,2-3天传一代 A类 C36 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose)  10%FBS L-M (TK-)细胞是L-M（ATCC CCL-1.2 ）细胞系的衍生株，具有抗5-溴脱氧尿核苷（5-bromo-2-deoxyuridine,BUdR）、缺乏胸腺嘧啶核苷激酶（thymidine kinase mutant）的特性，广泛用于遗传学、生物化学基础研究。也可用在体细胞杂交、基因转移等实验研究中。  L-M(TK-) 细胞 http://www.cellresource.cn/pdf/20080108100710.pdf 中国典型培养物保藏中心细胞库 同工酶鉴定 基础培养基+8%DMSO+20%FBS 小鼠结缔组织细胞胸苷激酶缺陷株；L-M(TK-) 成纤维细胞样 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性