EL4 cell EL4 细胞 NICR730 cell NICR730 细胞 3131C0001000800003 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. EL4 EL4 cell EL4 was established from lymphoma induced with 9,10-dimethyl-1,2-benzanthracene in C57BL mice. The cells are resistant to 0.1 mM hydrocortisone and sensitive to 20 mcg/ml PHA. There is a subline (EL4.IL-2, ATCC TIB-181) can produce high levels of IL-2. It is showed that the test for limb bone malformations virus (ectromelia virus) is negative. LDH检测报告EL4-Isoenzyme-SHCRC LDH检测报告EL4=6-Isoenzyme-SHCRC Mouse Lymphoma Cells; EL4 NICR730 Negative PN5 Passage at 1: 2. The cells can be overgrown within 3 days. RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). http://www.cellresource.cn/fdetail.aspx?id=730 http://www.cellresource.cn/pdf/20080108101040.pdf lymphoblast suspension growth 1:2。3天内可长满。 A类 EL4 细胞 EL4是从用9,10-二甲基-1,2-苯并蒽在C57BL小鼠中诱导的淋巴瘤中建立的。 能抗0.1 mM 氢化可的松，对20 mcg/ml PHA敏感。 还有一个亚株(EL4.IL-2, ATCC TIB-181)可以生成高水平的IL-2。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 LDH检测报告EL4-同工酶-SHCRC LDH检测报告EL4=6-同工酶-SHCRC PN5 RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 小鼠淋巴瘤细胞；EL4 悬浮生长 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 淋巴母细胞 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 阴性