P388D1 (IL-1) cell P388D1 (IL-1) 细胞 NICR235 cell NICR235 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000235 55~65 Base Medium+8%DMSO+20%FBS C4 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Mouse Lymphoma Cells; P388D1(IL-1) NICR235 P388D1(IL-1) P388D1(IL-1) cell Passage at 1: 4 ~ 1: 8; and change fluid for 3 times a week RPMI 1640 (w/o Hepes) 20%HS；4.5g/L Glucose The cells are derived from lymphoma induced by methylcholanthrene; they can produce IL-1 and lysozyme under the induction of LPS and phorbol ester; they can engulf yeast polysaccharide and latex microspheres, and are active in antibody-dependent, cell-mediated cytotoxic system. The cells are surface immunoglobulin (sIg)-negative and ectromelia virus-negative. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth http://www.cellresource.cn/fdetail.aspx?id=235 http://www.cellresource.cn/pdf/20080108101040.pdf round - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:4~1:8传代；每周换液3次。 55~65 A类 C4 P388D1(IL-1) 细胞 RPMI 1640 (w/o Hepes) 20%HS；4.5g/L Glucose http://www.cellresource.cn/pdf/20080108101040.pdf 中国医学科学院基础医学研究所基础医学细胞中心 圆形 培养法（-） 基础培养基+8%DMSO+20%FBS 小鼠淋巴瘤细胞；P388D1(IL-1) 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞来源于甲基胆蒽诱导形成的淋巴瘤；在LPS和佛波酯的诱导下可以产生IL-1，还可以产生溶菌酶；可以吞噬酵母多糖和乳胶微球，在抗体依赖的、细胞介导的细胞毒系统中有活性。表面免疫球蛋白（sIg）阴性，鼠痘病毒阴性。 贴壁生长