MC3T3-E1 Subclone 14 cell MC3T3-E1 Subclone 14 细胞 NICR503 cell NICR503 细胞 3131C0001000300015 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. MC3T3-E1 Subclone 14 MC3T3-E1 Subclone 14 cell MC3T3-E1 subclone 14-cell morphology-SHCRC Mouse parietal bone cell Subclone 14; MC3T3-E1 Subclone 14 NICR503 Negative P(15+5) The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). This cell is a series of subclones isolated from a cloned but differently phenotypic MC3T3-E1 cell line. A subclone of high or low osteoblast differentiation and mineralization was selected from osteoblasts grown in a medium containing ascorbic acid. Growth of MC3T3 subclone 4 (ATCC CRL-2593) and MC3T3 subclone 14 (ATCC CRL-2594) in ascorbic acid and 3 to 4 mM inorganic phosphate showed high levels of osteoblast differentiation. After 10 days, they formed a well-mineralized extracellular matrix (ECM). Growth of MC3T3 subclone 24 (ATCC CRL-2594) and MC3T3 subclone 30 (ATCC CRL-2596) in ascorbic acid showed poor osteoblast differentiation adherent growth fibroblasts http://www.cellresource.cn/fdetail.aspx?id=503 http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) qualified fetal bovine serum，10% α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 优质胎牛血清，10% A类 MC3T3-E1 Subclone 14 细胞 MC3T3-E1 subclone 14-细胞形态-SHCRC P(15+5) http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 优质胎牛血清，10% 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 小鼠颅顶前骨细胞亚克隆14；MC3T3-E1 Subclone 14 成纤维细胞 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 该细胞是从克隆的但是表型各异的MC3T3-E1细胞系中分离出一系列亚克隆。从含抗坏血酸培养基生长的成骨细胞中选择高或低成骨细胞分化、矿化的亚克隆。 MC3T3亚克隆4（ATCC CRL-2593）和MC3T3亚克隆14（ATCC CRL-2594）在抗坏血酸和3到4mM无机磷酸盐中生长表现出高水平的成骨细胞分化。 它们10天后形成一个矿化良好的细胞外基质（ECM）。MC3T3亚克隆24（ATCC CRL-2594）和MC3T3亚克隆30（ATCC CRL-2596）在抗坏血酸中生长表现出很差的成骨细胞分化 贴壁生长 阴性