OP9 cell OP9 细胞 NICR504 cell NICR504 细胞 3131C0001000300017 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion 5-10 minutes. Passage at 1: 2. The cells can be overgrowth within 3-4 days. Mouse Calvarial Fibroblasts; OP9 NICR504 Negative OP9 OP9 cell OP9 cell lines are derived from the cranium of newborn op/op mice. Because of a mutation in the gene encoding M-CSF, it does not produce a functional macrophage clone stimulating factor (M-CSF). The presence of M-CSF has an inhibitory function on the differentiation of embryonic stem cells (ES) into blood cells rather than other macrophages. OP9 cells can be used to co-culture with mouse embryonic stem cells to induce differentiation of embryonic stem cells into erythropoietic-derived, bone marrow-derived and B-cell lineage blood cells. Co-culture with OP9 does not require exogenous growth factors or complex embryonic structures. This system is useful for studying the molecular mechanism of the development and differentiation of hematopoietic cells. PN5 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth fibroblasts http://www.cellresource.cn/fdetail.aspx?id=504 http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) qualified fetal bovine serum，20% α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 优质胎牛血清，20% A类 OP9 细胞 OP9细胞株源自新生的op/op 小鼠颅盖。因编码M-CSF的基因中的一个突变，它不能生成有功能的巨噬细胞克隆刺激因子(M-CSF)。M-CSF的存在对胚胎干细胞(ES)分化成血细胞而不是其他巨噬细胞有抑制功能。 OP9细胞可以用于与小鼠胚胎干细胞共培养以诱导胚胎干细胞分化成成红血球来源的、骨髓来源的和B细胞谱系的血细胞。与OP9共培养不需要外源的生长因子或复杂的胚胎结构。这个系统对研究造血细胞的发育和分化的分子机理有用。 PN5 http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 优质胎牛血清，20% 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 小鼠颅盖成纤维细胞；OP9 成纤维细胞 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化5-10分钟。1:2。3-4天长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性