3T3-L1 cell 3T3-L1 细胞 NICR499 cell NICR499 细胞 3131C0001000300011 3T3-L1 3T3-L1 cell 3T3-L1 is a subline of continuous passages isolated from 3T3 cells (Swiss albino) by cloning. The cells undergo a transition from a rapidly split to confluence and contact-inhibiting state with the transition from preadipocyte to adipocyte. This cell is negative for ectromelia virus test, which produces triglycerides and high concentrations of serum can enhance intracellular fat accumulation. This cell line was introduced in 2003. 3T3-L1-cell morphology-SHCRC Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class B Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) bovine serum，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 牛血清，10% Mouse Embryonic Fibroblast; 3T3-L1 NICR499 Negative PN The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=499 http://www.cellresource.cn/pdf/20080108100951.pdf 3T3-L1 细胞 3T3-L1-细胞形态-SHCRC 3T3-L1是从3T3细胞（Swiss albino）中经克隆分离得到的连续传代的亚系。该细胞从快速分裂到汇合和接触性抑制状态经历了前脂肪细胞到脂肪样细胞的转变。该细胞鼠痘病毒阴性；可产生甘油三酯，高浓度血清可增强细胞内脂肪堆积。此细胞系为2003年引进 B类 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 牛血清，10% PN http://www.cellresource.cn/pdf/20080108100951.pdf 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 小鼠胚胎成纤维细胞；3T3-L1 成纤维细胞样 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性