3T3-L1 cell 3T3-L1 细胞 NICR1668 cell NICR1668 细胞 3131C0001000300025 3T3-L1 3T3-L1 cell 3T3-L1-cell morphology-SHCRC Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. L1 is a continuous substrain of 3T3 (swiss mouse) isolated by cloning. When cells split from rapid division to contact inhibition when overgrowing, the cells reverse from prefat to fatlike morphology. High serum content in culture solution can promote fat accumulation. This cell line was introduced in 2010. MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) bovine serum，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 牛血清，10% Mouse Embryonic Fibroblast; 3T3-L1 NICR1668 Negative PN5 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth fibroblasts http://www.cellresource.cn/fdetail.aspx?id=1668 http://www.cellresource.cn/pdf/20080108100951.pdf 3T3-L1 细胞 3T3-L1-细胞形态-SHCRC A类 L1是通过克隆分离得到的3T3 (swiss小白鼠)的连续亚株。 当细胞从快速分裂到长满且接触抑制时，该细胞经过前脂肪向脂肪样逆转。 培液中，高血清含量可以促进脂肪积累。此细胞系为2010年引进 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 牛血清，10% PN5 http://www.cellresource.cn/pdf/20080108100951.pdf 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 小鼠胚胎成纤维细胞；3T3-L1 成纤维细胞 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性