3T3-Swiss albino cell 3T3-Swiss albino 细胞 NICR1 cell NICR1 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000001 3T3-Swiss albino 3T3-Swiss albino cell 3T3-Swiss-Species Identification-PUMC Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Mouse Embryonic Fibroblast; 3T3-Swiss albino NICR1 Passage at 1: 3; and passage once in 3 to 4 days. The 3T3 cell line was established in 1962 by Todaro G and Green H from the isolated Swiss mouse embryo; the cells is contact inhibition, and cells in confluence was of 40 000 cells/cm2; The test results showed that the cell was negative for ectromelia virus. In plastic bottle, cells grow well, and may be in poor condition on some glass surface; the density may be about 50,000 cells/cm2 when the cell growth was saturated. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=1 http://www.cellresource.cn/pdf/20080108100710.pdf - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3传代；3~4天1次。 3T3-Swiss albino 细胞 3T3-Swiss-物种鉴定-PUMC 3T3细胞株是1962年Todaro G和Green H从分离的瑞士小鼠胚胎中建立的；该细胞的生长受接触性抑制，汇合状态的单层细胞密度为40000个细胞/平方厘米；检测结果显示该细胞鼠痘病毒阴性；在塑料瓶中生长较好，在某些玻璃表面上可能状态不佳；细胞生长饱和时其密度可以达到约50000 cells/cm2。 A类 C5 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf 中国医学科学院基础医学研究所基础医学细胞中心 培养法（-） 基础培养基+8%DMSO+20%FBS 小鼠胚胎成纤维细胞；3T3-Swiss albino 成纤维细胞样 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长