3T3-Swiss albino cell 3T3-Swiss albino 细胞 NICR411 cell NICR411 细胞 3111C0002000000039 3T3 swiss albino-cell morphology-NIFDC 3T3-Swiss albino 3T3-Swiss albino cell Base Medium+8%DMSO+20%FBS Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Mouse Embryonic Fibroblast; 3T3-Swiss albino NICR411 National Institute for Food and Drug Control Passage at 1: 3; and passage once in 3 to 4 days. Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net The 3T3 cell line was established in 1962 by Todaro G and Green H from the isolated Swiss mouse embryo; the cells is contact inhibition, and cells in confluence was of 40 000 cells/cm2; The test results showed that the cell was negative for ectromelia virus. In plastic bottle, cells grow well, and may be in poor condition on some glass surface; the density may be about 50,000 cells/cm2 when the cell growth was saturated. Unknown adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=411 http://www.cellresource.cn/pdf/20080108100710.pdf 1:3传代；3~4天1次。 3T3 swiss albino-细胞形态-NIFDC 3T3-Swiss albino 细胞 3T3细胞株是1962年Todaro G和Green H从分离的瑞士小鼠胚胎中建立的；该细胞的生长受接触性抑制，汇合状态的单层细胞密度为40000个细胞/平方厘米；检测结果显示该细胞鼠痘病毒阴性；在塑料瓶中生长较好，在某些玻璃表面上可能状态不佳；细胞生长饱和时其密度可以达到约50000 cells/cm2。 A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf 不详 中国食品药品检定研究院 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 小鼠胚胎成纤维细胞；3T3-Swiss albino 成纤维细胞样 贴壁生长