PA317 cell PA317 细胞 NICR260 cell NICR260 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 22~29;58~64 3111C0001CCC000260 Base Medium+8%DMSO+20%FBS C3 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Mouse Embryonic Fibroblast; PA317 NICR260 PA317 PA317 cell PA317 cells derived from TK-NIH/3T3 cells were constructed by co-transfection of the packaging structure DNA (pPAM3) in pBR322 and the herpes simplex virus thymidine kinase gene in pBR322. The introduction of retroviral vectors into these cells by infection or transfection results in the formation of amphotropic vector particles that can infect many mammalian cells. Viral particles generated from the cells have been successfully used to introduce genes into humans. The percentage of PA317 cells capable of packaging retroviral vectors decreases with passage, possibly due to the loss of transferred DNA used to construct this strain of cells. A brief (approximately 5 days) selection in media containing 0.03 mM hypoxanthine, 0.001 mM methotrexate and 0.02 mM thymidine can select the ability of retaining the packaging function. The screened cells should be cultured in HT medium for 4 days to dilute the residual methotrexate. PA317-Species Identification-PUMC PA317-cell morphology-PUMC Passage at 1: 10, and passage once in 5 to 10 days, and change fluid once in 2~3 days. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=260 http://www.cellresource.cn/pdf/20080108100710.pdf - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:10传代,5~10天一次，2~3天换液1次 22~29;58~64 A类 C3 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS PA317 细胞 PA317-物种鉴定-PUMC PA317-细胞形态-PUMC PA317细胞株源自TK- NIH/3T3细胞，通过pBR322中的包装结构DNA(pPAM3)和pBR322中的单纯疱疹病毒胸苷激酶基因共同转染构建而成。通过感染或转染将逆转录病毒载体导入这些细胞，生成可以感染许多哺乳动物细胞的双嗜性载体颗粒。这株细胞生成的病毒颗粒已成功地用于将基因导入人体。可能因为转入DNA丢失，能够包装逆转录病毒载体的PA317细胞百分比随传代而减少。在含0.03 mM 次黄嘌呤, 0.001 mM 氨甲喋呤和0.02 mM 胸苷的培养基中短暂(大约5天)选择，可以选出保留了包装功 http://www.cellresource.cn/pdf/20080108100710.pdf 中国医学科学院基础医学研究所基础医学细胞中心 培养法（-） 基础培养基+8%DMSO+20%FBS 小鼠胚胎成纤维细胞；PA317 成纤维细胞样 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长