BNL CL.2 cell BNL CL.2 细胞 NICR1665 cell NICR1665 细胞 3131C0001000300022 BNL CL-2-cell morpholgoy-SHCRC BNL CL.2 BNL CL.2 cell Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Mouse Embryo Hepatocytes; BNL CL.2 NICR1665 Negative P(9+5) The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). The cells were selected and established on plates in which arginine and tyrosine were substituted by ornithine and phenylalanine. Tests showed that the cells were limb bone dysplasia virus ectromelia virus-negative. adherent growth http://www.cellresource.cn/fdetail.aspx?id=1665 http://www.cellresource.cn/pdf/20080108100710.pdf A类 BNL CL-2-细胞形态-SHCRC BNL CL.2 细胞 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% P(9+5) http://www.cellresource.cn/pdf/20080108100710.pdf 中国科学院上海生命科学研究院细胞资源中心 在用鸟氨酸和苯丙氨酸代替精氨酸和酪氨酸的平板上选择建立了这株细胞。检测表明肢骨发育畸形病毒(鼠痘)阴性。 基础培养基+8%DMSO+20%FBS 小鼠胚胎肝细胞；BNL CL.2 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性