A-9 cell A-9 细胞 NICR500 cell NICR500 细胞 3131C0001000300012 A-9 A-9 cell A-9-cell morpholgoy-SHCRC A9 cell line was isolated from wild type cell line L. It is sensitive to HAT selection medium, and was deficient in denine phosphoribosyltransferase and hypoxanthine phosphoribosyl-transferase, and resistant to 8-azaguanine (0.003 mg/ml) and 6-thioguanine (0.1 mM). Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Mouse Subcutaneous Connective Tissue Cells; A-9 NICR500 Negative PN5 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth fibroblasts http://www.cellresource.cn/fdetail.aspx?id=500 http://www.cellresource.cn/pdf/20080108100710.pdf A-9 细胞 A-9-细胞形态-SHCRC A9细胞株由野生型细胞株L中分离得到。 对HAT选择培养基敏感，并缺失了腺苷转磷酸核糖基酶与次黄嘌呤转磷酸核糖基酶 对8-氮鸟嘌呤(0.003mg/ml)和6-硫代鸟嘌呤(0.1mM)有抗性。 A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% PN5 http://www.cellresource.cn/pdf/20080108100710.pdf 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 小鼠皮下结缔组织细胞；A-9 成纤维细胞 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性