4T1 cell 4T1 细胞 NICR755 cell NICR755 细胞 3131C0001000800032 4T1 4T1 cell 4T1-cell morphology-SHCRC Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Mouse Breast Cancer Cells; 4T1 NICR755 Negative PN5 RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% The 4T1 cell line is a 6-thioguanine resistant cell strain screened out from the 410.4 tumor strain without mutagenesis. When injected into BALB/c mice, 4T1 spontaneously produces a high metastatic tumor that can be transferred to lung, liver, lymph nodes and brain while creating an initial focal at the injection site. There is no need to extirpate the Initial focal during metastasis induction. The growth and metastasis of 4T1 cells in BALB/c mice were very similar to that in human breast cancer. This tumor is an animal model for stage VI human breast cancer. The kinetics of 4T1-induced tumor metastasis was similar in the postoperative and non-surgical conditions, and could be used as a postoperative and non-surgical model. Due to the anti-6-thioguanine properties of 4T1, small metastatic cell clusters (as few as 1) are detectable in many distal organs compared to other tumor models. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial cell http://www.cellresource.cn/fdetail.aspx?id=755 http://www.cellresource.cn/pdf/20080108101040.pdf 4T1 是从410.4瘤株中未经诱变筛得的6-硫鸟嘌噙抗性细胞株。 当注射到BALB/c 小鼠中时，4T1自发产生高转移肿瘤，可转移到肺，肝，淋巴结和大脑，同时在注射部位形成始发灶。 诱导转移时不需要摘除始发灶。 4T1细胞在BALB/c小鼠中的生长与转移特性与人体中的乳腺癌十分相近。这种肿瘤是人VI期乳腺癌的动物模型。 4T1-诱导的肿瘤在手术后及未手术情况下转移的动力学相近，可以用作手术后及未手术模型。 跟其他肿瘤模型相比，由于4T1的抗6-硫鸟嘌噙特性，微小的转移细胞团(少到仅仅1个)也可 4T1 细胞 4T1-细胞形态-SHCRC A类 PN5 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf 上皮细胞 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 小鼠乳腺癌细胞；4T1 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性