Neuro-2a cell Neuro-2a 细胞 NICR291 cell NICR291 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000291 Base Medium+8%DMSO+20%FBS C3 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS Mouse Neuroblastoma Tumor Cells; Neuro-2a [N2a; Neuro-2a] N2a N2a cell NICR291 Neural and amoebic-like stem cells Neuro-2a Neuro-2a cell Neuro-2a, established by Klebe RJ and Ruttle FH, is derived from spontaneous neuroblastoma in Albino A mice; it produces a large amount of tubulin that plays an important role in axoplasmatic action. The cell can be used to study the mechanism of vincristine precipitation of tubulin, the kinetics of GTP binding to the isolated protein, the flow of tubulin in vivo, and the synthesis and aggregation of tubulin. The OIE tissue is used to make routine diagnosis of rabies virus. Ectromelia virus test is negative. Neuro-2a-Species Identification-PUMC Passage at 1: 3 ~ 1: 6, and change fluid for 1 to 2 times a week. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth http://www.cellresource.cn/fdetail.aspx?id=291 http://www.cellresource.cn/pdf/20080108100951.pdf - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3~1:6传代，每周换液1~2次。 A类 C3 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS N2a 细胞 Neuro-2a 细胞 Neuro-2a 该细胞由Klebe RJ和Ruddle FH建立，来源于Albino A系小鼠的自发神经母细胞瘤；可产生大量的微管蛋白，该蛋白在对神经细胞的轴浆流动起主要作用的收缩系统中发挥重要作用。该细胞可用于研究长春新碱沉淀微管蛋白的机制、GTP与分离蛋白结合的动力学、体内微管蛋白的流动和微管蛋白的合成与聚集。OIE组织用该细胞做狂犬病的常规诊断。鼠痘病毒阴性。 Neuro-2a-物种鉴定-PUMC http://www.cellresource.cn/pdf/20080108100951.pdf 中国医学科学院基础医学研究所基础医学细胞中心 培养法（-） 基础培养基+8%DMSO+20%FBS 小鼠神经母细胞瘤细胞；Neuro-2a [N2a; Neuro-2a] 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 神经和阿米巴样干细胞 贴壁生长