M-1 cell M-1 细胞 NICR223 cell NICR223 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000223 Base Medium+8%DMSO+20%FBS C4 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS M-1 M-1 cell Mouse Renal Collection Tube (SV40 Transformation); M-1 NICR223 Passage at 1: 3 ~ 1: 4; and change fluid for 2~3 times a week. The cells are derived from the normal kidney tissue of tg (SV40E) Bri7 transgenic mice, containing the DNA sequence of SV40 virus, and retained some characteristics of the cortical manifold, such as morphology and CCD (cortical collecting duct) antigen. Most clones of M-1 cells have the characteristics of intercalated cells or principle cells in the cortical manifold, and 5% ～ 10% of the cells have double phenotype of intercalated cells and principle cells. When the cells are grown on a permeable support, a lumen-like structure with a negative transmembrane potential difference can be formed. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=223 http://www.cellresource.cn/pdf/20080108100542.pdf - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3~1:4传代；每周换液2~3次。 A类 C4 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS M-1 细胞 http://www.cellresource.cn/pdf/20080108100542.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 培养法（-） 基础培养基+8%DMSO+20%FBS 小鼠肾集合管细胞(SV40转化)；M-1 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞源于tg(SV40E)Bri7转基因小鼠的正常肾组织，含SV40病毒的DNA序列，保留了皮质集合管的某些特性，如形态和CCD（皮质结合管）抗原。大多M-1细胞的克隆具有皮质集合管中闰细胞或主细胞的特征；5%~10%的细胞有闰细胞和主细胞的双重表型。当该细胞在渗透性支持物上生长时，可形成具有负跨膜电位差的腔样结构。 贴壁生长