M5076 cell M5076 细胞 NICR287 cell NICR287 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000287 Base Medium+8%DMSO+20%FBS C3 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class B Culture Method（-） M5076 M5076 cell M5076-Cell Morphology-PUMC Mouse Reticular Cell Sarcoma; M5076 NICR287 Passage at 1: 2 ~ 1: 3, and passage once in 3 to 4 days. RPMI 1640 (w/o Hepes) 15% HS This cell is derived from a spontaneous reticulocyte sarcoma in C57BL/6 mouse ovary tissue and is identified as macrophage origins by cell morphology and function identification: It has phagocytosis activity; it expresses Fc receptor; it forms rosette with sheep erythrocytes; it has antibody-dependent cytotoxicity to red blood cells labeled with 51Cr; it has macrophage-like cytotoxicity to homologous mouse tumor cells(B16-F1) without the need for LPS activation; it has no NK cell activity; the activity of lysozyme, non-specific esterase and phosphatase is equivalent to that of mouse peritoneal macrophages; Ultrastructure showed that there were phagocytic vesicles in the cells, and no tight junction. The characteristics were consistent with the morphological characteristics of macrophages. Karyotype analysis showed that the cell was hypodiploid, because of Robertsonian translocation, >80% of the chromosomes Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com http://www.cellresource.cn/fdetail.aspx?id=287 http://www.cellresource.cn/pdf/20080108101040.pdf semi-suspension growth, adherent cells were short shuttle spindle - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:2~1:3传代，3~4天1次。 B类 C3 M5076 细胞 M5076-细胞形态-PUMC RPMI 1640 (w/o Hepes) 15% HS http://www.cellresource.cn/pdf/20080108101040.pdf 中国医学科学院基础医学研究所基础医学细胞中心 半悬浮生长，贴壁细胞呈短梭形。 培养法（-） 基础培养基+8%DMSO+20%FBS 小鼠网织细胞肉瘤；M5076 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 梭形 该细胞来源于C57BL/6小鼠卵巢组织自发的网织细胞肉瘤，经细胞形态和功能鉴定后为巨噬细胞来源：该细胞具有吞噬活性；表达Fc受体，与绵羊红细胞形成玫瑰花环；对51Cr标记的红细胞具有抗体依赖的细胞毒反应；对同源小鼠肿瘤细胞（B16-F1）有巨噬细胞样的细胞毒作用，且不需要LPS的激活；没有NK细胞的活性；具有同小鼠腹腔巨噬细胞相当的溶菌酶、非特异性酯酶和磷酸酶的活性；超微结构显示该细胞内有吞噬小泡，缺乏紧密连接，这些特点都符合巨噬细胞的形态特征。核型分析显示该细胞为亚二倍体，因罗伯逊易位，>80%的染色体