PK136 cell PK136 细胞 NICR560 cell NICR560 细胞 3131C0001000600046 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Hybridoma cell line PK136 secretes a mouse monoclonal antibody (IgG2a), which reacts with mouse NK cells. The cell line was established by the fusion of SP2/0-Ag14 myeloma and spleen cells of (C3H X BALB/C) F1 mice immunized with spleen cells and bone marrow cells of CE mice. This antibody is NK specific and cytotoxic in the presence of complement. The reaction pattern of this antibody with the cells of different mouse strains was consistent with that of the conventional NK1.1 antiserum. Mouse Hybridoma Cells; PK136 NICR560 Negative PK136 PK136 cell PN5 Passage at 1: 2. The cells can be overgrown within 3 days. RPMI 1640 (w/o Hepes) 10% FBS The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). http://www.cellresource.cn/fdetail.aspx?id=560 http://www.cellresource.cn/pdf/20080108101040.pdf lymphoblast-like suspension growth 1:2。3天内可长满。 A类 PK136 细胞 PN5 RPMI 1640 (w/o Hepes) 10% FBS http://www.cellresource.cn/pdf/20080108101040.pdf 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 小鼠杂交瘤细胞；PK136 悬浮生长 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 杂交瘤细胞系PK136分泌一种小鼠单克隆抗体（IgG2a），此抗体与小鼠NK细胞起反应。 该细胞系由SP2/0-Ag14骨髓瘤和用CE小鼠脾细胞和骨髓细胞免疫的（C3H X BALB/C）F1小鼠的脾细胞融合而成。 此抗体对NK特异且在有补体时有细胞毒作用。 此抗体与不同小鼠株的细胞的反应方式，与常规的NK1.1抗血清的反应方式相符。 淋巴母细胞样 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 阴性