expresses TM4 cell TM4 细胞 NICR298 cell NICR298 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000298 76~92 Base Medium+8%DMSO+20%FBS C6 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 5%HS + 2.5%FBS NICR298 Normal Mouse Testicular Leydig Cells; TM4 Passage at 1: 10 ~ 1:50; and change fluid once in 3~4 days. TM4 TM4 cell TM4-Species Identification-PUMC TM4-cell morphology-PUMC This cell increased cAMP production under the action of FSH, but did not respond to LH; compared to primary Sertoli cells, the reponse of the cells to FSH decreased. It is reported that the constitutively plasminogen activator production of the cell is very low, but can be stimulated by FSH, and tretinoin stimulation can have a significant increase in the production. The cells can produce retinol binding proteins, tissue plasminogen activator and transferrin, and express FSH receptor, androgen receptor and progesterone receptor. The cells are ectromelia virus-negative. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=298 http://www.cellresource.cn/pdf/20080108100542.pdf - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:10~1:50传代；3~4天换液1次。 76~92 A类 C6 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 5%HS + 2.5%FBS TM4 细胞 TM4-物种鉴定-PUMC TM4-细胞形态-PUMC http://www.cellresource.cn/pdf/20080108100542.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 正常小鼠睾丸Leydig细胞；TM4 该细胞在FSH的作用下cAMP产量增加，但对LH无反应；与原代Sertoli细胞相比，该细胞对FSH的反应性降低。据报道，该细胞组成性纤溶酶原激活物的产量很低，但可被FSH刺激产生，受维甲酸刺激可有大幅增高。该细胞可产生视黄醇结合蛋白、组织纤溶酶原激活物和转铁蛋白；表达FSH受体、雄激素受体和孕激素受体。鼠痘病毒阴性。 贴壁生长 androgen receptor progesterone receptor