CHO-K1 cell CHO-K1 细胞 NICR378 cell NICR378 细胞 3111C0002000000006 Base Medium+8%DMSO+20%FBS CHO-K1 CHO-K1 cell CHO-K1-cell morphology-NIFDC Chinese Hamster Ovary Cell K1 (Clonal Sublines); CHO-K1 Class A Culture Method（-） DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) new-born calf serum，10% DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 新生牛血清，10% In 1957, Puck TT established CHO cells from biopsies of adult Chinese hamster ovary, and CHO-K1 was a subclone of CHO. The growth of CHO-K1 requires proline. NICR378 National Institute for Food and Drug Control Passage at 1: 4-1: 8 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=378 http://www.cellresource.cn/pdf/20080108100542.pdf 1957年，Puck TT从成年中国仓鼠卵巢的活检组织建立了CHO细胞，CHO-K1是CHO的一个亚克隆。CHO-K1的生长需要脯氨酸。 1：4-1：8 A类 CHO-K1 细胞 CHO-K1-细胞形态-NIFDC DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 新生牛血清，10% http://www.cellresource.cn/pdf/20080108100542.pdf 上皮样 中国仓鼠卵巢细胞K1(亚系克隆)；CHO-K1 中国食品药品检定研究院 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 贴壁生长