CHO-K1 cell CHO-K1 细胞 NICR786 cell NICR786 细胞 3142C0001000000018 Base Medium+8%DMSO+20%FBS C420 CHO-K1 CHO-K1 cell Cell Library of China Center For Type Culture Collection Chinese Hamster Ovary Cell K1 (Clonal Sublines); CHO-K1 Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS In 1957, Puck TT established CHO cells from biopsies of adult Chinese hamster ovary, and CHO-K1 was a subclone of CHO. The growth of CHO-K1 requires proline. NICR786 Negative Passage at 1: 3, and passage once in 2-3 days. STR Identification The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=786 http://www.cellresource.cn/pdf/20080108100542.pdf 1957年，Puck TT从成年中国仓鼠卵巢的活检组织建立了CHO细胞，CHO-K1是CHO的一个亚克隆。CHO-K1的生长需要脯氨酸。 1:3传代,2-3天传一代 A类 C420 CHO-K1 细胞 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS STR鉴定 http://www.cellresource.cn/pdf/20080108100542.pdf 上皮细胞样 中国仓鼠卵巢细胞K1(亚系克隆)；CHO-K1 中国典型培养物保藏中心细胞库 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性