expresses NICR125 cell NICR125 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000125 7WPS1 7WPS1 cell Base Medium+8%DMSO+20%FBS C2 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；250ug/mlG418+2.5ug/ml puromycin Human APP-PS1 Double Genes Transfection CHO Cell Line; 7WPS1 NICR125 Passage at 1: 3; and passage once in 2 to 3 days. Wild-type PS1 gene was transfected into CHO cells stably expressing APP gene by liposome transfection and the cells expressed PS-1 and secreted Aβ. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth http://www.cellresource.cn/fdetail.aspx?id=125 http://www.cellresource.cn/pdf/20080108100710.pdf polygonal - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3传代；2~3天1次。 7WPS1 细胞 A类 C2 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；250ug/mlG418+2.5ug/ml puromycin http://www.cellresource.cn/pdf/20080108100710.pdf 中国医学科学院基础医学研究所基础医学细胞中心 人APP-PS1双基因转染CHO细胞株；7WPS1 利用脂质体转染法将野生型PS1基因转染稳定表达APP基因的CHO细胞，该细胞既表达PS-1，同时也分泌Aβ。 培养法（-） 基础培养基+8%DMSO+20%FBS 多角 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长 CHO derived cell line cell CHO衍生细胞系细胞 presenilin-1