NICR2059 cell NICR2059 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000440 Base Medium+8%DMSO+20%FBS Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class B Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 5%FBS + 1.0ug/mlpuromycin F9-CAG-tTA-3E5 F9-CAG-tTA-3E5 cell NICR2059 Passage at 1: 10, and passage once in 2~3 days. The plasmid pCAG-tTA was transfected into the cells, which was selected by 1.0 ug/ml puromysin, and the cells are Tet-off stable regulatory cell line, with the regulation fator of doxycycline Dox for more than 100 times. The cell doubling time was 24 hours. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth http://www.cellresource.cn/fdetail.aspx?id=2059 http://www.cellresource.cn/pdf/20080108100710.pdf polygonal tTA Gene-modified Mouse Testicular Teratocarcinoma Cells (Class B); F9-CAG-tTA-3E5 - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:10传代，2~3天传代一次。 B类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 5%FBS + 1.0ug/mlpuromycin F9-CAG-tTA-3E5 细胞 http://www.cellresource.cn/pdf/20080108100710.pdf tTA基因修饰的小鼠睾丸畸胎瘤细胞（B类）；F9-CAG-tTA-3E5 中国医学科学院基础医学研究所基础医学细胞中心 培养法（-） 基础培养基+8%DMSO+20%FBS 多角 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长 转染pCAG-tTA质粒，经1.0ug/ml puromycin筛选，为Tet-off稳定调控细胞系，受强力霉素Dox调控倍数达100倍以上。细胞倍增时间为24小时。 F9 derived cell line cell F9衍生细胞系细胞