NICR2512 cell NICR2512 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000591 Amelogenin：x,x；CSF1PO：10,12；D12S391：18,19；D13S317：11,11；D16S539：11,12；D18S51：16,20；D19S433：13,13；D21S11：30,31；D2S1338：18,19；D3S1358：15,15；D5S818：11,13；D6S1043：12,18；D7S820：9,11；D8S1179：13,14；FGA：21,21；Penta E：11,11；TH01：6,7；TPOX：8,11；vWA：18,18； Base Medium+8%DMSO+20%FBS C3 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Digestion 3-5 minutes; Passage at 1: 2 passage; The cells can be overgrowth within 3 days. HEC-1-B-Cas9-591 HEC-1-B-Cas9-591 cell Human Endometrial Adenocarcinoma Cells with Stable Expression of燙as9; HEC-1-B-Cas9-591 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；2ug/mlPuromycin NICR2512 Negative The cells made the HEC-1B cells stably express Cas9-Flag-Puromycin by lentivirus infection and was screened by 2ug/ml puromycin and then the monoclonal can be obtained. The expression of Casp9 in the monoclonal was confirmed by Western Blotting and the genomic DNA can be edited directly by Crispr technique. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=2512 http://www.cellresource.cn/pdf/20080108100951.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 Amelogenin：x,x；CSF1PO：10,12；D12S391：18,19；D13S317：11,11；D16S539：11,12；D18S51：16,20；D19S433：13,13；D21S11：30,31；D2S1338：18,19；D3S1358：15,15；D5S818：11,13；D6S1043：12,18；D7S820：9,11；D8S1179：13,14；FGA：21,21；Penta E：11,11；TH01：6,7；TPOX：8,11；vWA：18,18； A类 C3 Cas9稳定表达的人子宫内膜腺癌细胞；HEC-1-B-Cas9-591 HEC-1-B-Cas9-591 细胞 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；2ug/mlPuromycin http://www.cellresource.cn/pdf/20080108100951.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 消化3-5分钟；1:2传代；3天内可长满。 该细胞是采用慢病毒感染的方式使HEC-1B细胞稳定表达Cas9-Flag-Puromycin，经2ug/ml嘌呤霉素筛选得到的单克隆，经Western Blotting验证该克隆可以表达Cas9蛋白，可直接用Crispr技术编辑基因组DNA。 贴壁生长 阴性 HEC-1-B derived cell line cell HEC-1-B衍生细胞系细胞