immortal cell line cell 永生细胞系细胞 NICR1988 cell NICR1988 细胞 3142C0001000000337 BTO: BTO_0003350 BV-2 was founded in 1990 by E. Blasi. The cells were immortalized by retroviral-mediated transfection of v-raf/v-myc into mouse microglia. The cell line retains a variety of morphological, characterization, and functional characteristics of microglia. The results of immunohistochemistry for MAC1 and MAC2 are positive, and that for MAC3, glial fibrillary acidic protein and galactose are negative. BV2 BV2 cell Base Medium+8%DMSO+20%FBS C7 CVCL: CVCL_0182 Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS MCCL: MCC_0000073 Mouse Microglia; BV2 NICR1988 Negative Passage at 1: 3, and passage once in 2-3 days. STR Identification The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=1988 http://www.cellresource.cn/pdf/20080108100710.pdf 1:3传代,2-3天传一代 A类 BV-2由E.Blasi年建立于1990年。该细胞由小鼠小神经胶质细胞经逆转录病毒介导转染v-raf/v-myc获永生化。该细胞系保留有小神经胶质细胞多种形态，表征，和功能特征。免疫组化结果显示细胞为MAC1,MAC2阳性，而MAC3，胶质细胞原纤维酸性蛋白，半乳糖脑苷脂为阴性。 BV2 细胞 C7 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS STR鉴定 http://www.cellresource.cn/pdf/20080108100710.pdf 上皮细胞样 中国典型培养物保藏中心细胞库 基础培养基+8%DMSO+20%FBS 小鼠小胶质细胞；BV2 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性