immortal cell line cell 永生细胞系细胞 NICR312 cell NICR312 细胞 - - - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000312 Base Medium+8%DMSO+20%FBS Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Cells were prepared into cell suspension and inoculated into Km and other mice. Class A Culture Method（-） It was established in 1985; the lines containing 20-methylcholanthrene were passed through cancer induced by removal of cervical tissue in fresh Kunming(KM) mouse, and the tumor cell suspension was transplanted and passaged subcutaneously in Kunming (Km) mouse; the success rate was 82%; it was well differentiated keratinized squamous cell carcinoma. KM Mouse Cervical Cancer Cell Lines; U27 NICR312 U27 U27 cell Unknown Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com growth in vivo http://www.cellresource.cn/fdetail.aspx?id=312 solid tumor - - - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1985年建立；含20-甲基胆蒽的线穿过新鲜昆明种幼鼠摘除子宫颈组织诱发癌后，瘤细胞悬液昆明种（Km）小鼠皮下移植及传代；移植成功率82%；为分化较好的角化鳞状细胞癌。 A类 KM小鼠子宫颈癌瘤株；U27 U27 细胞 不详 中国医学科学院基础医学研究所基础医学细胞中心 体内生长 制备成细胞悬液接种至Km等小鼠。 培养法（-） 基础培养基+8%DMSO+20%FBS 实体瘤 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com