immortal cell line cell 永生细胞系细胞 NICR87 cell NICR87 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000087 52~56 Amelogenin：X；CSF1PO：10；D13S317：13.3，15.1；D16S539：9，10；D18S51：16；D19S433：13；D21S11：27，28；D2S1338：17；D3S1358：15，18；D5S818：11，12；D7S820：12；D8S1179：12；FGA：18，21；TH01：7；TPOX：12；vWA：16，18； BTO: BTO_0003227 Base Medium+8%DMSO+20%FBS C5 CVCL: CVCL_0534 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Hepatocellular Carcinoma Cells; SMMC-7721 Human hepatoma tissue were collected, and the cell were cultured for 11 days by static and rotating tube method, and the cells was passaged for the first time after growing for 23 days. The cells are AFP-positive. The expression of 1.3 kb LFIRE-1/HFREP-1 mRNA in the cells could not be detected by Northern blot, which are tumorigenesis in vivo in immune deficient mice. STR detection showed that cell lines, such as SGC7901, SMMC7721, QGY7701, QGY7703, QSG7701, Ho-8910PM, Ho-8910, BEL7402 and so on, were in the same genetic background and are suspected of cross-contamination within each other. MCCL: MCC_0000433 NICR87 Passage at 1: 3; and passage once in 3 to 4 days. RPMI 1640 (w/o Hepes) 10%FBS SMMC-7721 SMMC-7721 cell SMMC7721-Cell Morphology-PUMC SMMC7721-Species Identification-PUMC SMMC7721-cell morphology-PUMC Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=87 http://www.cellresource.cn/pdf/20080108101040.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3传代；3~4天1次。 52~56 Amelogenin：X；CSF1PO：10；D13S317：13.3，15.1；D16S539：9，10；D18S51：16；D19S433：13；D21S11：27，28；D2S1338：17；D3S1358：15，18；D5S818：11，12；D7S820：12；D8S1179：12；FGA：18，21；TH01：7；TPOX：12；vWA：16，18； A类 C5 RPMI 1640 (w/o Hepes) 10%FBS SMMC-7721 细胞 SMMC7721-物种鉴定-PUMC SMMC7721-细胞形态-PUMC http://www.cellresource.cn/pdf/20080108101040.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人肝癌细胞；SMMC-7721 取人肝癌组织，采用静置和旋转管法培养11天细胞开始生长，首次传代23天。AFP阳性。用Northern blot方法，未能检测到细胞中1.3kb LFIRE-1/HFREP-1 mRNA的表达，免疫缺陷小鼠体内可成瘤。STR检测发现SGC7901、SMMC7721、QGY7701、QGY7703、QSG7701、Ho-8910PM、Ho-8910、BEL7402等多株细胞为同一遗传背景，怀疑彼此间存在交叉污染。 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长