immortal cell line cell 永生细胞系细胞 NICR613 cell NICR613 细胞 3131C0001000700043 Amelogenin:X；CSF1PO:10；D13S317:13.3；D16S539:9,10；D18S51:16；D19S433:13；D21S11:27,28；D2S1338:17；D3S1358:15,18；D5S818:11,12；D7S820:12；D8S1179:12；FGA:18,21；TH01:7；TPOX:12；vWA:16,18 BTO: BTO_0005721 Base Medium+8%DMSO+20%FBS CVCL: CVCL_6715 Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen Unit: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences Zip code: 200032 Address: No. 320, Yueyang Road, Shanghai Tel: 021-54920404，021-54920405 Fax: 021-54920653 Email: shchen@sibs.ac.cn Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Human Hepatocellular Carcinoma Cells; QGY-7703 [QGY7703] NICR613 Negative PN QGY-7703 QGY-7703 cell QGY7703 QGY7703 cell RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). The cell line is from the liver cancer of a 35-year-old woman. The number of chromosomes varies greatly, and heteroploid is in the majority. Indirect immunofluorescence assay for AFP is positive. The ability for allograft is strong. In the aspect of submicroscopic structure, the ratio of nucleu/cytoplasm is high, with large polymorphic nucleus and various organelles change in submicroscopic structure. The cells aggregate by the action of Con A. The doubling time is about 20.5 hours. The expression of 1.3 kb LFIRE-1/HFREP-1 mRNA has not been detected by Northern blot. adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=613 http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X；CSF1PO:10；D13S317:13.3；D16S539:9,10；D18S51:16；D19S433:13；D21S11:27,28；D2S1338:17；D3S1358:15,18；D5S818:11,12；D7S820:12；D8S1179:12；FGA:18,21；TH01:7；TPOX:12；vWA:16,18 A类 PN QGY-7703 细胞 QGY7703 细胞 RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf 上皮细胞样 中国科学院上海生命科学研究院细胞资源中心 人肝癌细胞；QGY-7703 [QGY7703] 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920653 Email: shchen@sibs.ac.cn 该细胞系来自35岁女性的肝癌。 染色体数目变化大，异倍体多。 免疫荧光间接法AFP阳性反应。 异体移植能力强。 亚显微结构方面，核与细胞质的比例高，大的多形核和多种细胞器亚显微结构改变。 在Con A作用下凝集。倍增时间约为20.5小时。 用Northern blot方法，未能检测到细胞中1.3kb LFIRE-1/HFREP-1 mRNA的表达。 贴壁生长 阴性