immortal cell line cell 永生细胞系细胞 NICR803 cell NICR803 细胞 3142C0001000000035 BEL-7402 cell line was established from surgical specimens of clinical liver cancer in 1974. The population doubling time was 20 hours. Tumor nodules can be formed by transplantation in treated wistar rats. The cells were epithelioid-like cells. Under the electron microscope, the epithelial cells were also shown to have desmosomes and tensinogen fibers, similar to those found in clinical hepatocarcinoma cells. On the fourth day of bottle culture, the mitotic index was about 7%. The chromosome number of BEL-7402 cells is less than triploid, and there is an abnormal acrocentric chromosome. The AFP expression activity in BEL-7402 cells was positive in indirect immunofluorescence assay. LDH isoenzyme spectrum of the cells was different from that of adult liver cells, but similar to human embryo liver and clinical liver cancer. BEl-7402 BEl-7402 cell BTO: BTO_0004050 Base Medium+8%DMSO+20%FBS C261 CVCL: CVCL_5492 Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Human Hepatocellular Carcinoma Cells; BEl-7402 Isoenzyme Identification Chromosome NICR803 Negative Passage at 1: 3, and passage once in 2-3 days. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=803 http://www.cellresource.cn/pdf/20080108100710.pdf 实验生物学报，11（1）：37-45,1978 1:3传代,2-3天传一代 A类 BEL-7402细胞株是1974年从临床肝癌手术标本中建立的。 细胞群体倍增时间为20小时。 移植到处理过的wistar大鼠中的能形成肿瘤结节。 细胞形态呈上皮样细胞。 在电子显微镜下亦显示上皮细胞所具有的桥粒和张力原纤维，与临床肝癌细胞相似。 分瓶培养第四天时，其有丝分裂指数约为7%。 BEL-7402细胞的染色体数为不足三倍体，有一个异常的近端着丝点染色体。 间接免疫荧光法测BEL-7402细胞内的AFP为阳性反应。 LDH同工酶谱显示与成年人肝细胞不同，而与人胚肝及临床肝癌相近。 BEl-7402 细胞 C261 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf 上皮细胞样 中国典型培养物保藏中心细胞库 人肝癌细胞；BEl-7402 同工酶鉴定 基础培养基+8%DMSO+20%FBS 实验生物学报，11（1）：37-45,1978 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性