immortal cell line cell 永生细胞系细胞 NICR831 cell NICR831 细胞 1.Wang JX, Liu GH, Fan YZ, et al.Effects of cytotoxic T lymphocytes on hepatoma cell line SMMC-7721 induced by different subsets of dendritic cells in vitro. Hepatobiliary Pancreat Dis Int, 2006, 5: 422-427. 3142C0001000000063 BTO: BTO_0003227 Base Medium+8%DMSO+20%FBS C65 CVCL: CVCL_0534 Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Human Hepatocellular Carcinoma Cells; SMMC-7721 Human hepatoma tissue were collected, and the cell were cultured for 11 days by static and rotating tube method, and the cells was passaged for the first time after growing for 23 days. The cells are AFP-positive. The expression of 1.3 kb LFIRE-1/HFREP-1 mRNA in the cells could not be detected by Northern blot, which are tumorigenesis in vivo in immune deficient mice. Isoenzyme Identification Chromosome MCCL: MCC_0000433 NICR831 Negative Passage at 1: 3, and passage once in 2-3 days. SMMC-7721 SMMC-7721 cell The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=831 http://www.cellresource.cn/pdf/20080108100710.pdf 1.Wang JX, Liu GH, Fan YZ, et al.Effects of cytotoxic T lymphocytes on hepatoma cell line SMMC-7721 induced by different subsets of dendritic cells in vitro. Hepatobiliary Pancreat Dis Int, 2006, 5: 422-427. 1:3传代,2-3天传一代 A类 C65 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS SMMC-7721 细胞 http://www.cellresource.cn/pdf/20080108100710.pdf 上皮细胞样 中国典型培养物保藏中心细胞库 人肝癌细胞；SMMC-7721 取人肝癌组织，采用静置和旋转管法培养11天细胞开始生长，首次传代23天。AFP阳性。用Northern blot方法，未能检测到细胞中1.3kb LFIRE-1/HFREP-1 mRNA的表达，免疫缺陷小鼠体内可成瘤。 同工酶鉴定 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性