immortal cell line cell 永生细胞系细胞 NICR638 cell NICR638 细胞 3131C0001000700070 Amelogenin:X,Y；CSF1PO:12；D13S317:10；D16S539:11,12；D18S51:13,17；D19S433:14；D21S11:29,32.2；D2S1338:23,24；D3S1358:15,16；D5S818:11,15；D7S820:11；D8S1179:10,11；FGA:21,23；TH01:9；TPOX:8；vWA:14,17 Base Medium+8%DMSO+20%FBS CVCL: CVCL_6909 Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. HCE8693 HCE8693 cell Hce-8693 Hce-8693 cell Hce-8693 originated from a pathological section of lymph node metastasis in a male patient with caecal undifferentiated adenocarcinoma. Its doubling time is 30.4 hours and mitotic index is 28.8%. Giant nuclei is observed by electron microscopy, in which nucleolar demarcation was obvious, with abundant microfilaments and secretory granules. The number of chromosome patterns is 48. The ratio of cloning on soft agar is 8%. Test for CEA is positive in the supernatant of cell neutralization culture medium. When transplanted into nude mice, Hce8693 cells grow into the same tumor as the patient's original focus, with alcian blue-positive materials in the cytoplasm. Hce-8693-cell morphology-SHCRC Human Undifferentiated Cecum Adenocarcinoma Cells; Hce-8693 [HCE8693] NICR638 Negative PN5 RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=638 http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X,Y；CSF1PO:12；D13S317:10；D16S539:11,12；D18S51:13,17；D19S433:14；D21S11:29,32.2；D2S1338:23,24；D3S1358:15,16；D5S818:11,15；D7S820:11；D8S1179:10,11；FGA:21,23；TH01:9；TPOX:8；vWA:14,17 A类 HCE8693 细胞 Hce-8693 细胞 Hce-8693-细胞形态-SHCRC Hce-8693源自一位男性盲肠未分化腺癌患者的淋巴结转移的病理切片。它的倍增时间是30.4小时，有丝分裂指数是28.8%。 电子显微镜检查呈现巨核，核仁分界明显，微丝丰富，并有分泌颗粒。染色体模式数是48。 在软琼脂上成克隆的比率是8%。 细胞中和培养液上清中CEA阳性。 当异体移植到裸鼠时，Hce8693细胞长成的瘤与患者原病灶一样，在细胞质中有阿尔新蓝阳性物质。 PN5 RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf 上皮样 中国科学院上海生命科学研究院细胞资源中心 人盲肠腺癌细胞（未分化）；Hce-8693 [HCE8693] 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性