immortal cell line cell 永生细胞系细胞 NICR861 cell NICR861 细胞 3142C0001000000093 BIU-87 BIU-87 cell BIU-87 was established in 1989 (Yu Lizhang et al.). The cell line is derived from human papillary transitional cell carcinoma of bladder, and 0.2 ml of 5 � 108 cells are usually inoculated subcutaneously in nude mice, representing progressive tumor growth, while the detection of tumor sarcoidosis for the cells is similar to that of the original cancer specimen. The population doubling time of the 22 generations was 34 hours. The cells could grow on soft agar at a cloning efficiency of 23%. BIU-87 cell line has agglutination reaction to ConA, WGA and PSL. Base Medium+8%DMSO+20%FBS C115 CVCL: CVCL_6881 Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn Human Bladder Cancer Cells; BIU-87 Isoenzyme Identification Chromosome NICR861 Negative Passage at 1: 3, and passage once in 2-3 days. RPMI 1640 (w/o Hepes) 10%FBS The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=861 http://www.cellresource.cn/pdf/20080108101040.pdf 1:3传代,2-3天传一代 A类 BIU-87 细胞 BIU-87建系于1989年（俞莉章等）。该细胞系来源于人膀胱乳头状移行上皮癌，通常采用5×108细胞0.2ml接种裸鼠皮下，呈进行性肿瘤生长，肿瘤结节病检与原标本癌组织相似。22代细胞群体倍增时间为34小时。细胞能在软琼脂上生长，克隆形成率23%。BIU-87细胞系对ConA，WGA，PSL均有凝集反应。 C115 RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 上皮细胞样 中国典型培养物保藏中心细胞库 人膀胱癌细胞；BIU-87 同工酶鉴定 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性