immortal cell line cell 永生细胞系细胞 NICR296 cell NICR296 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000296 Amelogenin：X；CSF1PO：9，10；D13S317：12，13.3；D16S539：9，10；D18S51：16；D19S433：13，14；D21S11：27，28；D2S1338：17；D3S1358：15，18；D5S818：11，12；D7S820：8，12；D8S1179：12，13；FGA：21；TH01：7；TPOX：8，12；vWA：16，18； Base Medium+8%DMSO+20%FBS CVCL: CVCL_6851 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Tongue Squamous Carcinoma Cells; Tca-8113 [Tca8113] NICR296 Passage at 1: 3 ~ 1: 4, and passage once in 2 to 3 days. RPMI 1640 (w/o Hepes) 20%FBS Tca-8113 Tca-8113 cell Tca-8113 tissue is derived from tongue squamous cell carcinoma in situ; the cells began to grow after blocks culture for 7 days, and the first passage was of 71 days and now 160 passages has been passaged with a passage time of 38 hours; On the 3rd day of passage, the mitotic index was 61%, the transplantation efficiency was 86%, the soft agar clone formation rate was 53% -57.7%. Subcutaneous transplantation of immunosuppressed C57BL/6 and ICR pups was successful, and the electron microscopic and histochemical characteristics were consistent with those of squamous cell carcinoma. STR detection revealed that the cells were contaminated by HeLa cells. Tca-8113-Species Identification-PUMC Tca8113 Tca8113 cell Unknown Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=296 http://www.cellresource.cn/pdf/20080108101040.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3~1:4传代，2~3天1次。 Amelogenin：X；CSF1PO：9，10；D13S317：12，13.3；D16S539：9，10；D18S51：16；D19S433：13，14；D21S11：27，28；D2S1338：17；D3S1358：15，18；D5S818：11，12；D7S820：8，12；D8S1179：12，13；FGA：21；TH01：7；TPOX：8，12；vWA：16，18； A类 RPMI 1640 (w/o Hepes) 20%FBS Tca-8113 细胞 Tca-8113-物种鉴定-PUMC Tca-8113源于原位舌鳞癌的活检组织；组织块培养法培养7天开始生长，首次传代71天，已传160代；传代时间为38小时；传代第3天有丝分裂指数为61%，移植效率为86%，软琼脂克隆生成率为53%~57.7%。免疫抑制的C57BL/6和ICR幼小鼠皮下移植成功；电镜和组化特征与鳞癌相符。STR检测发现该细胞被HeLa细胞污染。 Tca8113 细胞 http://www.cellresource.cn/pdf/20080108101040.pdf 上皮样 不详 中国医学科学院基础医学研究所基础医学细胞中心 人舌鳞癌细胞；Tca-8113 [Tca8113] 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长