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    <!-- http://purl.obolibrary.org/obo/CLO_0000019 -->

    <Class rdf:about="http://purl.obolibrary.org/obo/CLO_0000019">
        <rdfs:label xml:lang="en">immortal cell line cell</rdfs:label>
        <rdfs:label xml:lang="zh">永生细胞系细胞</rdfs:label>
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    <Class rdf:about="http://purl.obolibrary.org/obo/CLO_0053150">
        <rdfs:label xml:lang="en">NICR645 cell</rdfs:label>
        <rdfs:label xml:lang="zh">NICR645 细胞</rdfs:label>
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0000019"/>
        <ns2:IAO_0000119 xml:lang="en">1.费继敏, 李梅, 蒋永新, 等. 人舌鳞癌TCA8113细胞在不同温度下nm23-H_1基因的表达及其意义. 口腔颌面外科杂志, 2008, 18(2): 98-102.
2.王开, 康非吾, 闵睿, 等. Tca-8113 细胞系单细胞体外增殖的实验观察. 口腔颌面外科杂志, 2008, 18(6): 398-401.
3. 何荣根, 徐秀祺, 周晓健, 等. 人舌鳞状细胞癌Tca8113细胞系的建立及其生物学特性. 肿瘤，1983，(3): 97. </ns2:IAO_0000119>
        <ns2:CLO_0051622 xml:lang="en">3131C0001000700077</ns2:CLO_0051622>
        <ns2:CLO_0051629 xml:lang="en">Base Medium+8%DMSO+20%FBS</ns2:CLO_0051629>
        <rdfs:seeAlso xml:lang="en">CVCL: CVCL_6851</rdfs:seeAlso>
        <ns2:CLO_0051636 xml:lang="en">Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences</ns2:CLO_0051636>
        <ns2:CLO_0051633 xml:lang="en">Class A</ns2:CLO_0051633>
        <ns2:CLO_0051637 xml:lang="en">Contacts: Songhua Chen
Unit: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences
Zip code: 200032
Address: No. 320, Yueyang Road, Shanghai
Tel: 021-54920404，021-54920405
Fax: 021-54920711
Email: shchen@sibs.ac.cn</ns2:CLO_0051637>
        <ns2:CLO_0051638 xml:lang="en">Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days.</ns2:CLO_0051638>
        <ns2:CLO_0051623 xml:lang="en">Human Tongue Squamous Carcinoma Cells; Tca-8113 [Tca8113]</ns2:CLO_0051623>
        <ns2:IAO_0000118 xml:lang="en">NICR645</ns2:IAO_0000118>
        <ns2:CLO_0051630 xml:lang="en">Negative</ns2:CLO_0051630>
        <ns2:CLO_0051628 xml:lang="en">PN5</ns2:CLO_0051628>
        <ns2:CLO_0051627 xml:lang="en">RPMI 1640 (w/o Hepes) qualified fetal bovine serum，20%</ns2:CLO_0051627>
        <ns2:CLO_0051627 xml:lang="en">RPMI 1640 (w/o Hepes) 优质胎牛血清，20%</ns2:CLO_0051627>
        <ns2:CLO_0051626 xml:lang="en">TCA-8113 is derived from biopsy sections of carcinoma of the tongue in situ belonging to grade I squamous cell carcinoma (T2N1Amo, II stage). It was established in 1987 by dry adherent method, and the passage time was 38 hours. On the 3rd day of passage, the mitotic index was 61%, the transplantation efficiency was 86%, the soft agar clone formation rate was 53% -57.7%. The cells form tumors in ICR and B5 mice after ATS treatment. The characteristics of electron microscope and histochemistry were consistent with squamous cell carcinoma.</ns2:CLO_0051626>
        <ns2:IAO_0000118 xml:lang="en">Tca-8113</ns2:IAO_0000118>
        <ns2:IAO_0000118 xml:lang="en">Tca-8113 cell</ns2:IAO_0000118>
        <ns2:IAO_0000118 xml:lang="en">Tca8113</ns2:IAO_0000118>
        <ns2:IAO_0000118 xml:lang="en">Tca8113 cell</ns2:IAO_0000118>
        <rdfs:comment xml:lang="en">The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains).</rdfs:comment>
        <ns2:CLO_0051625 xml:lang="en">adherent growth</ns2:CLO_0051625>
        <ns2:CLO_0051624 xml:lang="en">epithelial-like</ns2:CLO_0051624>
        <rdfs:seeAlso xml:lang="en">http://www.cellresource.cn/fdetail.aspx?id=645</rdfs:seeAlso>
        <ns2:CLO_0051627 xml:lang="en">http://www.cellresource.cn/pdf/20080108101040.pdf</ns2:CLO_0051627>
        <ns2:IAO_0000119 xml:lang="zh">1.费继敏, 李梅, 蒋永新, 等. 人舌鳞癌TCA8113细胞在不同温度下nm23-H_1基因的表达及其意义. 口腔颌面外科杂志, 2008, 18(2): 98-102.
2.王开, 康非吾, 闵睿, 等. Tca-8113 细胞系单细胞体外增殖的实验观察. 口腔颌面外科杂志, 2008, 18(6): 398-401.
3. 何荣根, 徐秀祺, 周晓健, 等. 人舌鳞状细胞癌Tca8113细胞系的建立及其生物学特性. 肿瘤，1983，(3): 97. </ns2:IAO_0000119>
        <ns2:CLO_0051633 xml:lang="zh">A类</ns2:CLO_0051633>
        <ns2:CLO_0051628 xml:lang="zh">PN5</ns2:CLO_0051628>
        <ns2:CLO_0051627 xml:lang="zh">RPMI 1640 (w/o Hepes) 优质胎牛血清，20%</ns2:CLO_0051627>
        <ns2:IAO_0000118 xml:lang="zh">Tca-8113 细胞</ns2:IAO_0000118>
        <ns2:CLO_0051626 xml:lang="zh">Tca-8113源自属于I级鳞癌(T2N1Amo, II stage)的原位舌癌的活组织检查切片。 通过干贴壁方法建立于1987年，传代时间为38小时。 传代第三天有丝分裂指数为61%，移植效率为86%，软琼脂克隆生成率为53-57.7%。 ATS处理后在ICR和C57B1小鼠中成瘤。 电镜和组化特征与鳞癌相符。</ns2:CLO_0051626>
        <ns2:IAO_0000118 xml:lang="zh">Tca8113 细胞</ns2:IAO_0000118>
        <ns2:CLO_0051627 xml:lang="zh">http://www.cellresource.cn/pdf/20080108101040.pdf</ns2:CLO_0051627>
        <ns2:CLO_0051624 xml:lang="zh">上皮细胞样</ns2:CLO_0051624>
        <ns2:CLO_0051636 xml:lang="zh">中国科学院上海生命科学研究院细胞资源中心</ns2:CLO_0051636>
        <ns2:CLO_0051623 xml:lang="zh">人舌鳞癌细胞；Tca-8113 [Tca8113]</ns2:CLO_0051623>
        <ns2:CLO_0051629 xml:lang="zh">基础培养基+8%DMSO+20%FBS</ns2:CLO_0051629>
        <rdfs:comment xml:lang="zh">本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。</rdfs:comment>
        <ns2:CLO_0051638 xml:lang="zh">消化3-5分钟。1:2。3天内可长满。</ns2:CLO_0051638>
        <ns2:CLO_0051637 xml:lang="zh">联系人：陈松华
单位：中国科学院上海生命科学研究院细胞资源中心
邮编：200032
地址：上海市岳阳路320号
电话：021-54920404，021-54920405
传真：021-54920711
Email: shchen@sibs.ac.cn</ns2:CLO_0051637>
        <ns2:CLO_0051625 xml:lang="zh">贴壁生长</ns2:CLO_0051625>
        <ns2:CLO_0051630 xml:lang="zh">阴性</ns2:CLO_0051630>
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