immortal cell line cell 永生细胞系细胞 NICR236 cell NICR236 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000236 60~82 Amelogenin：X；CSF1PO：10；D13S317：13.3；D16S539：9，10；D18S51：16；D19S433：13；D21S11：27，28；D2S1338：17；D3S1358：15，18；D5S818：11，12；D7S820：12；D8S1179：12；FGA：18，21；TH01：7；TPOX：12；vWA：16，18； BTO: BTO_0002392 Base Medium+8%DMSO+20%FBS C5 CVCL: CVCL_0520 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） EFO: EFO_0006293 Human Gastric Cancer Cells; SGC-7901 [SGC7901] It was established in 1979; the cell line was originated from a lymph node metastasis of a 56-year-old woman with gastric adenocarcinoma. The cell line was cultured in RPMI-1640 for 12 days and began to grow, and the first passage was of 10 days; the cell line was passaged to 186 generations within 31 months. The cells were were transplanted subcutaneously to immunosuppressive ICR mice and the suckling dogs successfully, and transplanted to the anterior chamber of rabbits and suckling dogs transplantation successfully; the lymph node metastasis invaded from mice subcutaneous tissue to muscular layer. The STR detection showed that cell lines, such as SGC7901, SMMC7721, QGY7701, QGY7703, QSG7701, Ho - 8910PM, Ho - 8910, BEL7402 and so on, were in the same genetic background and suspected of cross contamination within each other. NICR236 Passage at 1: 3; and passage once in 2 to 3 days. RPMI 1640 (w/o Hepes) 10%FBS SGC-7901 SGC-7901 cell SGC7901 SGC7901 cell SGC7901-Cell Morphology-PUMC SGC7901-Species Identification-PUMC Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=236 http://www.cellresource.cn/pdf/20080108101040.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1979年建系；这株细胞源自一位56岁女性胃腺癌患者的淋巴结转移灶。RPMI-1640培养12天，细胞开始生长，首次传代10天；31个月中传代186代。免疫抑制的ICR小鼠、乳犬皮下移植成功，家兔、乳犬眼前房移植成功；淋巴结转移，从小鼠皮下侵袭至肌层。STR检测发现SGC7901、SMMC7721、QGY7701、QGY7703、QSG7701、Ho-8910PM、Ho-8910、BEL7402等多株细胞为同一遗传背景，怀疑彼此间存在交叉污染。 1:3传代；2~3天1次。 60~82 Amelogenin：X；CSF1PO：10；D13S317：13.3；D16S539：9，10；D18S51：16；D19S433：13；D21S11：27，28；D2S1338：17；D3S1358：15，18；D5S818：11，12；D7S820：12；D8S1179：12；FGA：18，21；TH01：7；TPOX：12；vWA：16，18； A类 C5 RPMI 1640 (w/o Hepes) 10%FBS SGC-7901 细胞 SGC7901 细胞 SGC7901-物种鉴定-PUMC SGC7901-细胞形态-PUMC http://www.cellresource.cn/pdf/20080108101040.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人胃腺癌细胞；SGC-7901 [SGC7901] 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长