mortal cell line cell 非永生细胞系细胞 NICR899 cell NICR899 细胞 3142C0001000000131 Base Medium+8%DMSO+20%FBS C150 Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn Mouse Mononuclear-macrophage Leukemia Cells; RAW NICR899 Negative Passage at 1: 3, and passage once in 2-3 days. RAW RAW cell RPMI 1640 (w/o Hepes) 10%FBS STR Identification The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). This cell line is derived from a tumor induced by Abelson murine leukemia virus in BALB/c mice. The cells produce Lysozyme can be produced, and detections for sIg-, Ia-antigen and Thy-1.2 surface antigen are negative. To detect the secretion of viral particles, the XC spot formation assay is negative. The cells could drink neutral red and devour latex particles and zymosan, and could divide sheep red blood cells and tumor target cells in an antibody-dependent manner; LPS or PPD treatment for 2 days can induce split of red blood cells but had no effect on tumor target cells. adherent growth http://www.cellresource.cn/fdetail.aspx?id=899 http://www.cellresource.cn/pdf/20080108101040.pdf lymphoblast-like 1:3传代,2-3天传一代 A类 C150 RAW 细胞 RPMI 1640 (w/o Hepes) 10%FBS STR鉴定 http://www.cellresource.cn/pdf/20080108101040.pdf 中国典型培养物保藏中心细胞库 基础培养基+8%DMSO+20%FBS 小鼠单核巨噬细胞白血病细胞；RAW 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 此细胞株源自BALB/c小鼠由Abelson鼠科白血病病毒诱导的肿瘤。可产生溶菌酶；sIg-, Ia-，Thy-1.2-。为检测到病毒颗粒的分泌，XC斑点形成试验阴性。该细胞可以胞饮中性红并吞噬乳胶颗粒与酵母聚糖；可以经抗体依赖分裂绵羊红细胞与肿瘤靶细胞；LPS或PPD处理2天可诱导分裂红细胞，但对肿瘤靶细胞无作用。 淋巴母细胞样 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性