immortal cell line cell 永生细胞系细胞 NICR193 cell NICR193 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000193 55~64 Base Medium+8%DMSO+20%FBS C3 CVCL: CVCL_G363 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） It was established in May 1979; derived from spontaneous papillary lung adenocarcinoma of inbred T739 Inbred mice. In primary culture, the cells began to grow after 24 hours, and the first passage was of 45 days and epithelial-like round cells, adherent growth, then the cells were transplanted to T739, 615 and Km mice successfully; the cells were transplanted subcutaneously with 100% success rate. LA795 LA795 cell LA795-cell morphology-PUMC Mouse Lung Adenocarcinoma Cell Line; LA795 NICR193 Passage at 1: 6; and passage once in 2 days. RPMI 1640 (w/o Hepes) 10%FBS Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=193 http://www.cellresource.cn/pdf/20080108101040.pdf - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1979年5月建系；取自T739近交系小鼠自发乳头型肺腺癌。原代培养建系，24小时细胞开始生长，首次传代45天,上皮样圆形细胞，贴壁生长 ，T739、615、Km小鼠皮下移植成功；T739小鼠肺100%转移。 1:6传代；2天1次。　 55~64 A类 C3 LA795 细胞 LA795-细胞形态-PUMC RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 培养法（-） 基础培养基+8%DMSO+20%FBS 小鼠肺腺癌细胞系；LA795 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长