mortal cell line cell 非永生细胞系细胞 NICR79 cell NICR79 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000079 BTO: BTO_0002572 Base Medium+8%DMSO+20%FBS CSEO: CSEO_00001496 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%CS EFO: EFO_0004040 MEF MEF cell Mouse Embryonic Fibroblast; MEF NICR79 P1 Passage at 1: 3; and passage once in 3 to 4 days. The 615 mouse embryos at 9 days of pregnancy were cultured and established by removing brain and heart. The cells can be used as feeder layer cells to support the growth of embryonic stem cells ES and maintain an undifferentiated state of ES. When MEF is used as feeder cell, MEF should be treated with mitomycin C to stop growth. It is recommended that MEF should not exceed 6 passages as feeder layer of ES cells. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=79 http://www.cellresource.cn/pdf/20080108100710.pdf - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3传代；3~4天1次。 A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%CS MEF 细胞 P1 http://www.cellresource.cn/pdf/20080108100710.pdf 中国医学科学院基础医学研究所基础医学细胞中心 取孕9天的615小鼠胚胎，去除脑、心脏等培养建立。该细胞可用作饲养层细胞，支持胚胎干细胞ES的生长并维持ES未分化的状态。当作为饲养层细胞时，MEF需经丝裂霉素C处理停止生长。建议作为ES细胞的饲养层时，MEF不要超过6代。 培养法（-） 基础培养基+8%DMSO+20%FBS 小鼠胚胎成纤维细胞；MEF 成纤维细胞样 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长