immortal cell line cell 永生细胞系细胞 NICR2710 cell NICR2710 细胞 15%FBS, 1000U/ml LIF,3 μM CHIR99021, and 1 μM PD0325901 3111C0001CCC000597 Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College Knockout-DMEM+10%DMSO+20%FBS Murine Embryonic Stem Cells NICR2710 Negative in Microbiological Culture, PCR Assays Passage at 1:3, passage 2-3 days PiggyBac transporon based homozygous rights, arrayed, colons, 96-well format The experimental scheme to generate the arrayed mutant haploid ES cell libraries Zhenli Yang，Yuqin Liu, Tel:65286441，69156455；Email:ccc1@ibms.pumc.edu.cn adherent dome-shaped colonies http://www.cellresource.cn/fdetail.aspx?id=2710 mouse mutant haploid embryonic stem cell libraries mouse mutant haploid embryonic stem cell libraries cell 15%FBS, 1000U/ml LIF,3 μM CHIR99021, and 1 μM PD0325901 Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College Knockout-DMEM+10%DMSO+20%FBS The experimental scheme to generate the arrayed mutant haploid ES cell libraries Zhenli Yang，Yuqin Liu, Tel:65286441，69156455；Email:ccc1@ibms.pumc.edu.cn adherent dome-shaped colonies mouse mutant haploid embryonic stem cell libraries 细胞 negative in microbiological culture, PCR assays passage at 1:3, passage 2-3 days piggyBac transposon based homozygous mutant libraries， arrayed， colonies， 96-well format 小鼠胚胎干细胞 mouse mutant haploid embryonic stem cell libraries