immortal cell line cell 永生细胞系细胞 NICR63 cell NICR63 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000063 57~66 BTO: BTO_0003350 BV2 BV2 cell BV2-Species Identification-PUMC BV2-cell morphology-PUMC Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS It is derived from C57BL/6 mice microglia, expressing nuclear v-myc, v-raf oncogene, and express env gp70 antigen on the surface. It has the characteristics of morphology, phenotype and function of phagocytes. Mouse Microglia; BV2 NICR63 PMID: 12429020 PMID: 14646166 PMID: 15471861 Passage at 1: 6; and passage once in 2 to 3 days. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com http://www.cellresource.cn/fdetail.aspx?id=63 http://www.cellresource.cn/pdf/20080108100710.pdf pleomorphic shape semi-adherent growth - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:6传代；2~3天1次。 57~66 A类 BV2 细胞 BV2-物种鉴定-PUMC BV2-细胞形态-PUMC C5 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS http://www.cellresource.cn/pdf/20080108100710.pdf 中国医学科学院基础医学研究所基础医学细胞中心 半贴壁生长 培养法（-） 基础培养基+8%DMSO+20%FBS 多形型 小鼠小胶质细胞；BV2 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 源于C57BL/6小鼠小胶质细胞，表达核v-myc、染色体v-raf 癌基因，表面表达env gp70抗原，在形态学、表型及功能上有吞噬细胞的特征。