HEK293-derived cell 293衍生细胞 NICR127 cell NICR127 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000127 AMS2(SCF3) AMS2(SCF3) cell Base Medium+8%DMSO+20%FBS Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Stem Cell Factor Monoclonal Antibody Cell Line; AMS2(SCF3) NICR127 Passage at 1: 3; and passage once in 2 to 3 days. RPMI 1640 (w/o Hepes) 10%FBS The cell line was established by Professor Chen Songsen. The c-Kit/Ig1 ~ 3 gene (Human stem cell factor receptor (c-Kit) ligand binding domain extracellular N-terminal 1-3 immunoglobulin-like domain) with histidine tag added to C-terminal was cloned into the eukaryotic expression vector pEAK12, and transfected to 293ET cell line and screened for the cell line with high expression of c-kit/Ig1～3. The expression level of the cell culture supernatant was 2 μg/ml and the relative molecular weight of the fusion protein was 58 000; The receptor ligand binding assay showed that the cells expressed c-Kit/Ig1 ~ 3, which had specific binding activity to stem cell factor. Unknown Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com http://www.cellresource.cn/fdetail.aspx?id=127 http://www.cellresource.cn/pdf/20080108101040.pdf lymphoblast-like semi-adherent growth 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3传代；2~3天1次。 AMS2(SCF3) 细胞 A类 RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 不详 中国医学科学院基础医学研究所基础医学细胞中心 人干细胞因子单克隆抗体细胞株；AMS2(SCF3) 半贴壁生长 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 淋巴母细胞样 该细胞株由陈松森教授实验室建立；将C端添加组氨酸标签的c-Kit/Ig1～3基因（人干细胞因子受体(c-Kit)配体结合区膜外N端1～3免疫球蛋白样结构域）克隆入真核表达载体pEAK12中,转染293ET细胞中筛选得到c-kit/Ig1～3高效表达细胞株。该细胞培养上清表达量为2μg/ml,融合蛋白相对分子质量为58 000；受体配体结合实验显示该细胞表达的c-Kit/Ig1～3有明显的干细胞因子特异结合活性。