NICR636 cell NICR636 细胞 3131C0001000700068 A new human megakaryocyte (Dami) was established from the blood of a patient with megakaryocytic leukemia. The cells grew mainly in suspension with a doubling time of 24-30 hours. At least 89% of the cells are reactive with platelet glycoprotein (GP) lb, Ilb/llla and blood group glycoprotein monoclonal antibodies. It does not react with antibodies against lymphocytes, monocytes, granulocytes and macrophage antigens. Dami cells provide an excellent model for studying the biochemistry and differentiation of megakaryocytes. Amelogenin:X；CSF1PO:10；D13S317:9,11；D16S539:11；D18S51:12,16；D19S433:11,13；D21S11:29,31.2；D2S1338:18,19；D3S1358:15；D5S818:11；D7S820:7；D8S1179:13,15；FGA:22,23；TH01:7；TPOX:11；vWA:14,17 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Dami Dami cell Human Megakaryoblastic Leukemia Cells; Dami NICR636 Negative PN5 Passage at 1: 2. The cells can be overgrown within 3 days. RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). http://www.cellresource.cn/fdetail.aspx?id=636 http://www.cellresource.cn/pdf/20080108101040.pdf megakaryocyte semi-adherent growth 1:2。3天内可长满。 Amelogenin:X；CSF1PO:10；D13S317:9,11；D16S539:11；D18S51:12,16；D19S433:11,13；D21S11:29,31.2；D2S1338:18,19；D3S1358:15；D5S818:11；D7S820:7；D8S1179:13,15；FGA:22,23；TH01:7；TPOX:11；vWA:14,17 A类 Dami 细胞 PN5 RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf 中国科学院上海生命科学研究院细胞资源中心 人巨核细胞白血病细胞；Dami 从一个巨核细胞白血病患者的血液建立了一株新的人类巨核细胞(Dami)。 此细胞悬浮生长为主，倍增时间24-30小时。 至少89%的细胞可与血小板糖蛋白(GP) lb and Ilb/llla及血型糖蛋白单克隆抗体反应。 不与抗淋巴腺、单核细胞、粒性白细胞、巨噬细胞抗原的抗体反应。 Dami细胞为研究巨核细胞生化及分化提供了极好的模型。 半贴壁生长 基础培养基+8%DMSO+20%FBS 巨核细胞 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 阴性 HEL derived cell line cell HEL衍生细胞系细胞