NICR1660 cell NICR1660 细胞 3131C0001000200039 A human umbilical vein cell line, EA. Hy926, was constructed by fusion of primary cultured human umbilical vein cells with the thiopurine-resistant A549 cell line under PEG stress. The fusion genes were grown on HAT medium and screened by factor VIII. EA. Hy926 has been population doubling(PDLs) for more than 100 times. Electron microscopic photographs show that the cytoplasmic distribution of Weibel-Palade body as well as tissue-specific organelles, differentiated endothelial cell characteristics such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammatory reactions.[PubMed: 6407019] [PubMed:2079463] Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. EA.hy926 EA.hy926 cell EAhy926-cell morphology-SHCRC Human Umbilical Vein Cells Fusion Cells; EA.hy926 NICR1660 Negative PN5 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth endotheliocyte http://www.cellresource.cn/fdetail.aspx?id=1660 http://www.cellresource.cn/pdf/20080108100710.pdf A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% EA.hy926 细胞 EAhy926-细胞形态-SHCRC PN5 http://www.cellresource.cn/pdf/20080108100710.pdf 中国科学院上海生命科学研究院细胞资源中心 人脐静脉细胞融合细胞；EA.hy926 内皮细胞 在PEG胁迫下，将原代培养的人脐静脉细胞与抗硫鸟嘌呤的A549克隆株融合，构建了人脐静脉细胞株, EA.hy926。 融合子在HAT培养基上生长并以八因子相关抗原筛选。 EA.hy926已经过100次以上的群体倍增(PDLs)。 电镜照片显示Weibel-Palade小体的细胞质分布以及组织特异性的细胞器，分化的内皮细胞特性如血管生成，体内平衡/血栓形成，血压及炎症反应。 [PubMed: 6407019] [PubMed: 2079463] 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性 A549 derived cell line cell A549衍生细胞系细胞