NICR2148 cell NICR2148 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000475 A human umbilical vein cell line, EA. Hy926, was constructed by fusion of primary cultured human umbilical vein cells with the thiopurine-resistant A549 cell line under PEG stress. The fusion genes were grown on HAT medium and screened by factor VIII. EA. Hy926 has been population doubling(PDLs) for more than 100 times. Electron microscopic photographs show that the cytoplasmic distribution of Weibel-Palade body as well as tissue-specific organelles, differentiated endothelial cell characteristics such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammatory reactions. Amelogenin：X；CSF1PO：10,11,12；D13S317：11；D16S539：11，12；D18S51：13,15,17；D19S433：13,14；D21S11：28,29,32；D2S1338：22,24；D3S1358：15,16；D5S818：11；D7S820：8,9,10；D8S1179：13；FGA：22,23；TH01：6,8,9.3；TPOX：8,9；vWA：14,17； Base Medium+8%DMSO+20%FBS Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS EA-HY926-Species Identification-CRC EA.hy926 EA.hy926 cell Human Umbilical Vein Cells Fusion Cells; EA.hy926 NICR2148 Passage at 1: 2; and passage once in 2-3 days. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth http://www.cellresource.cn/fdetail.aspx?id=2148 http://www.cellresource.cn/pdf/20080108100710.pdf spindle 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:2传代；2-3天传代一次。 Amelogenin：X；CSF1PO：10,11,12；D13S317：11；D16S539：11，12；D18S51：13,15,17；D19S433：13,14；D21S11：28,29,32；D2S1338：22,24；D3S1358：15,16；D5S818：11；D7S820：8,9,10；D8S1179：13；FGA：22,23；TH01：6,8,9.3；TPOX：8,9；vWA：14,17； A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS EA-HY926-物种鉴定-CRC EA.hy926 细胞 http://www.cellresource.cn/pdf/20080108100710.pdf 中国医学科学院基础医学研究所基础医学细胞中心 人脐静脉细胞融合细胞；EA.hy926 在PEG胁迫下，将原代培养的人脐静脉细胞与抗硫鸟嘌呤的A549克隆株融合，构建了人脐静脉细胞株, EA.hy926。 融合子在HAT培养基上生长并以八因子相关抗原筛选。 EA.hy926已经过100次以上的群体倍增(PDLs)。 电镜照片显示Weibel-Palade小体的细胞质分布以及组织特异性的细胞器，分化的内皮细胞特性如血管生成，体内平衡/血栓形成，血压及炎症反应。 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 梭形 贴壁生长 A549 derived cell line cell A549衍生细胞系细胞