HEK293-derived cell 293衍生细胞 NICR2187 cell NICR2187 细胞 3142C0001000000950 Base Medium+8%DMSO+20%FBS Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn Human Primary Embryonic Kidney Transformed Cells; Trex-293 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS + Blasticidin NICR2187 Negative PN5 Passage at 1: 3, and passage once in 2-3 days. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). The cells were modified on the basis of 293 cells, and the tetracycline stably expressed in the medium containing mitogen was repressed, and the yield of the cell with recombinant adenovirus was higher than that of other cells. Trex-293 Trex-293 cell Trex-293-Myoplasma Detection-CCTCC Trex-293-cell morphology-CCTCC adherent growth epithelium-like; polygon http://www.cellresource.cn/fdetail.aspx?id=2187 http://www.cellresource.cn/pdf/20080108100951.pdf 1:3传代,2-3天传一代 A类 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS + Blasticidin PN5 Trex-293 细胞 Trex-293-支原体检测-CCTCC Trex-293-细胞形态-CCTCC http://www.cellresource.cn/pdf/20080108100951.pdf 上皮样；多角 中国典型培养物保藏中心细胞库 人原胚肾转化细胞；Trex-293 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 该细胞在293细胞的基础上进行了改造，在含有灭瘟素的培养基中稳定表达四环素阻遏，且重组腺病毒产率高于其他细胞的产率。 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性