HEK293 HEK293 cell NICR10 cell NICR10 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 293 293 cell 3111C0001CCC000010 Amelogenin：X；CSF1PO：7，12；D13S317：12；D16S539：9，13；D18S51：17，18；D19S433：15，18；D21S11：28，30.2；D2S1338：19；D3S1358：15，17；D5S818：8，9；D7S820：11；D8S1179：12，14；FGA：23；TH01：7，9.3；TPOX：11；vWA：16，19； Base Medium+8%DMSO+20%FBS Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Early reports indicated that the cell genome contained DNA of the left and right end of the adenovirus 5 (Ad5) genome, but it is now clear that only the DNA at the left end is present. By cloning and sequencing the insertion point of Ad5, it was found that the 1 ~ 4344 linear nucleotide of Ad5 was integrated into the cell chromosome 19q13.2. The cells are hosts for amplification of human adenovirus vectors. The cell surface receptor, which can express abnormal vitronectin, is composed of integrin β1 subunit and vitronectin receptor α-v subunit. The biological security level is Class 2. HEK-293 HEK-293 cell HEK293-Cell Morphology-PUMC HEK293-Species Identification-PUMC Human Embryonic Kidney Cells; 293 [HEK-293] MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS NICR10 P20 PMID: 16850753 Passage at 1: 10 ~ 1: 20, and change fluid once every 2~3 day. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=10 http://www.cellresource.cn/pdf/20080108100951.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:10~1:20传代，每2~3天换液1次。 293 细胞 Amelogenin：X；CSF1PO：7，12；D13S317：12；D16S539：9，13；D18S51：17，18；D19S433：15，18；D21S11：28，30.2；D2S1338：19；D3S1358：15，17；D5S818：8，9；D7S820：11；D8S1179：12，14；FGA：23；TH01：7，9.3；TPOX：11；vWA：16，19； A类 HEK-293 细胞 HEK293-物种鉴定-PUMC HEK293-细胞形态-PUMC MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS P20 PMID: 16850753 http://www.cellresource.cn/pdf/20080108100951.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人胚肾细胞；293 [HEK-293] 培养法（-） 基础培养基+8%DMSO+20%FBS 早期报道中指出该细胞基因组中含有腺病毒5（Ad5）基因组的左侧端和右侧端的DNA，但是现在明确了只存在其左侧端的DNA。经过对Ad5的插入点的克隆测序发现，Ad5的1～4344位线性核苷酸整合入细胞染色体19q13.2。该细胞为人类腺病毒载体扩增的宿主。可表达异常的玻连蛋白的细胞表面受体，由整合素β1亚单位和玻连蛋白受体α-v亚单位组成。生物安全级别为2级。 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长