HEK293 HEK293 cell NICR377 cell NICR377 细胞 293 293 cell 3111C0002000000005 Amelogenin:X；CSF1PO:11,12；D13S317:12,14；D16S539:9,13；D18S51:17,18；D21S11:28,30.2；D3S1358:15,17；D5S818:8,9；D7S820:11,12；D8S1179:12,14；FGA:23；PentaD:9,10；PentaE:7,15；TH01:7,9.3；TPOX:11；vWA:16,19 Base Medium+8%DMSO+20%FBS Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) new-born calf serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 新生牛血清，10% Early reports indicated that the cell genome contained DNA of the left and right end of the adenovirus 5 (Ad5) genome, but it is now clear that only the DNA at the left end is present. By cloning and sequencing the insertion point of Ad5, it was found that the 1 ~ 4344 linear nucleotide of Ad5 was integrated into the cell chromosome 19q13.2. The cells are hosts for amplification of human adenovirus vectors. The cell surface receptor, which can express abnormal vitronectin, is composed of integrin β1 subunit and vitronectin receptor α-v subunit. The biological security level is Class 2. HEK-293 HEK-293 cell HEK293-cell morphology-NIFDC Human B,NP,G9PG,MD,AST Human Embryonic Kidney Cells; 293 [HEK-293] NICR377 National Institute for Food and Drug Control P35 Passage at 1: 2-1: 4 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=377 http://www.cellresource.cn/pdf/20080108100710.pdf 1：2-1：4 293 细胞 Amelogenin:X；CSF1PO:11,12；D13S317:12,14；D16S539:9,13；D18S51:17,18；D21S11:28,30.2；D3S1358:15,17；D5S818:8,9；D7S820:11,12；D8S1179:12,14；FGA:23；PentaD:9,10；PentaE:7,15；TH01:7,9.3；TPOX:11；vWA:16,19 A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 新生牛血清，10% HEK-293 细胞 HEK293-细胞形态-NIFDC P35 http://www.cellresource.cn/pdf/20080108100710.pdf 上皮样 中国食品药品检定研究院 人B,NP,G9PG,MD,AST 人胚肾细胞；293 [HEK-293] 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 早期报道中指出该细胞基因组中含有腺病毒5（Ad5）基因组的左侧端和右侧端的DNA，但是现在明确了只存在其左侧端的DNA。经过对Ad5的插入点的克隆测序发现，Ad5的1～4344位线性核苷酸整合入细胞染色体19q13.2。该细胞为人类腺病毒载体扩增的宿主。可表达异常的玻连蛋白的细胞表面受体，由整合素β1亚单位和玻连蛋白受体α-v亚单位组成。生物安全级别为2级。 贴壁生长