HEK293-derived cell 293衍生细胞 NICR477 cell NICR477 细胞 293 Cells, low passage 293 Cells, low passage cell 293 cells, low passage-cell morphology-SHCRC 3131C0001000200018 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Human Embryonic Kidney Cells; 293 Cells, low passage MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 NICR477 Negative P(27+5) The 293 cells are human embryonic kidney cells transformed with spliced adenovirus 5 DNA. They contain and express the early segment 1 of adenovirus 5. They contain and express the early segment 1 of adenovirus 5. They can complement each other in E1 inactivated adenoviral mutants and vectors to allow their growth. They are particularly effective receptor cells in DNA transfection assays. For most of the growth and plaque tests, even if not involved in all human adenovirus, they perform well. These cells are widely used in the amplication and titration of E1 inactivated mutants caused by deletion or substitution of E1 sequences， and adenoviral vectors. Hence, they are use to construct adenoviral vectors. It is preferred to use low-passage cells for the construction of adenoviral vectors, not more than 40 generations in general. it is correct to maintain the excellent performance of this cell line in this way. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial cell http://www.cellresource.cn/fdetail.aspx?id=477 http://www.cellresource.cn/pdf/20080108100951.pdf 293 Cells, low passage 细胞 293 细胞, 低传代-细胞形态-SHCRC 293细胞是人胚胎肾细胞用剪切过的腺病毒5 DNA转化得到。它们包含并表达腺病毒5的早期区段1。 它们包含并表达腺病毒5的早期区段1。 它们能互补E1失活的腺病毒突变株和载体，使其生长。 在DNA转染检测中它们是特别有效的受体细胞。 对于大多数即使不是全部人腺病毒的生长和溶菌斑测试，它们也表现很好。 这些细胞广泛应用于因缺失或代换E1序列引起的E1失活突变株和腺病毒载体的繁殖与滴定。 因此它们被用来构建腺病毒载体。 用于构建腺病毒载体最好使用低代数细胞，一般不超过40代。 对于保持这株细胞的优良特性，正确 A类 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 P(27+5) http://www.cellresource.cn/pdf/20080108100951.pdf 上皮细胞 中国科学院上海生命科学研究院细胞资源中心 人胚肾细胞；293 Cells, low passage 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性