A-375 cell A-375 细胞 NICR1991 cell NICR1991 细胞 3142C0001000000340 A-375 A-375 cell A375 A375 cell Base Medium+8%DMSO+20%FBS C10 Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Human Malignant Melanoma Mells; A375[A-375] NICR1991 Negative Passage at 1: 3, and passage once in 2-3 days. STR Identification The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). The cells are derived from the skin tissue of a 54-year-old female melanoma patient, established by Giard DJ. The chromosome is hypertriploid and has 62 chromosomes. Among them, nine marker chromosomes are ubiquitous in A375 cells, while normal chromosomes N2, N6 and N22 are only one in A375 cells. adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=1991 http://www.cellresource.cn/pdf/20080108100710.pdf 1:3传代,2-3天传一代 A-375 细胞 A375 细胞 A类 C10 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS STR鉴定 http://www.cellresource.cn/pdf/20080108100710.pdf 中国典型培养物保藏中心细胞库 人恶性黑色素瘤细胞；A375[A-375] 基础培养基+8%DMSO+20%FBS 成纤维细胞样 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 该细胞源于一位54岁女性黑色素瘤患者的皮肤组织，由Giard DJ建系。其染色体为超三倍体，带有62条染色体。其中九条标志染色体普遍存在于A375细胞中，而普通染色体N2, N6和N22在细胞中只有一条。 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性