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    <!-- http://purl.obolibrary.org/obo/CLO_0001591 -->

    <Class rdf:about="http://purl.obolibrary.org/obo/CLO_0001591">
        <rdfs:label xml:lang="en">A431 cell</rdfs:label>
        <rdfs:label xml:lang="zh">A431 细胞</rdfs:label>
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        <rdfs:label xml:lang="en">NICR92 cell</rdfs:label>
        <rdfs:label xml:lang="zh">NICR92 细胞</rdfs:label>
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0001591"/>
        <rdfs:comment xml:lang="en">1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment.</rdfs:comment>
        <ns2:CLO_0051622 xml:lang="en">3111C0001CCC000092</ns2:CLO_0051622>
        <ns2:CLO_0051632 xml:lang="en">72~79</ns2:CLO_0051632>
        <ns2:IAO_0000118 xml:lang="en">A-431</ns2:IAO_0000118>
        <ns2:IAO_0000118 xml:lang="en">A-431 cell</ns2:IAO_0000118>
        <ns2:IAO_0000118 xml:lang="en">A431</ns2:IAO_0000118>
        <ns2:IAO_0000118 xml:lang="en">A431 cell</ns2:IAO_0000118>
        <ns2:CLO_0051635 xml:lang="en">A431-Cell Morphology-PUMC</ns2:CLO_0051635>
        <ns2:CLO_0051635 xml:lang="en">A431-Species Identification-PUMC</ns2:CLO_0051635>
        <ns2:CLO_0051635 xml:lang="en">A431-cell morphology-PUMC</ns2:CLO_0051635>
        <ns2:CLO_0037192 xml:lang="en">Amelogenin：X；CSF1PO：11，12；D13S317：9，13；D16S539：12，14；D18S51：13，17；D19S433：15，15.2；D21S11：28；D2S1338：17，20；D3S1358：14，20；D5S818：12，13；D7S820：10；D8S1179：13；FGA：20；TH01：9；TPOX：11；vWA：15，17；</ns2:CLO_0037192>
        <ns2:CLO_0051629 xml:lang="en">Base Medium+8%DMSO+20%FBS</ns2:CLO_0051629>
        <ns2:CLO_0051636 xml:lang="en">Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences</ns2:CLO_0051636>
        <ns2:CLO_0051633 xml:lang="en">Class A</ns2:CLO_0051633>
        <ns2:CLO_0051630 xml:lang="en">Culture Method（-）</ns2:CLO_0051630>
        <ns2:CLO_0051627 xml:lang="en">DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS</ns2:CLO_0051627>
        <ns2:CLO_0051623 xml:lang="en">Human Skin Squamous Cell Carcinoma Cells; A-431 [A431]</ns2:CLO_0051623>
        <ns2:IAO_0000118 xml:lang="en">NICR92</ns2:IAO_0000118>
        <ns2:CLO_0051628 xml:lang="en">P40</ns2:CLO_0051628>
        <ns2:IAO_0000119 xml:lang="en">PMID: 8552591</ns2:IAO_0000119>
        <ns2:CLO_0051638 xml:lang="en">Passage at 1: 3 ~ 1: 8; and change fluid once every 2~3 day.</ns2:CLO_0051638>
        <ns2:CLO_0051626 xml:lang="en">The cell, which originated from an 85-year-old woman with cutaneous squamous cell carcinoma, is one of a series of cell lines established by Giard DJ et al. The cells can form tumors in immunosuppressed mice and can be cultured on agar to form clones; the cell line is a hypertriploid human cell strain.</ns2:CLO_0051626>
        <ns2:CLO_0051637 xml:lang="en">Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com</ns2:CLO_0051637>
        <ns2:CLO_0051625 xml:lang="en">adherent growth</ns2:CLO_0051625>
        <ns2:CLO_0051624 xml:lang="en">epithelioid</ns2:CLO_0051624>
        <rdfs:seeAlso xml:lang="en">http://www.cellresource.cn/fdetail.aspx?id=92</rdfs:seeAlso>
        <ns2:CLO_0051627 xml:lang="en">http://www.cellresource.cn/pdf/20080108100710.pdf</ns2:CLO_0051627>
        <rdfs:comment xml:lang="zh">1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害</rdfs:comment>
        <ns2:CLO_0051638 xml:lang="zh">1:3~1:8传代；每2~3天换液1次。</ns2:CLO_0051638>
        <ns2:CLO_0051632 xml:lang="zh">72~79</ns2:CLO_0051632>
        <ns2:IAO_0000118 xml:lang="zh">A-431 细胞</ns2:IAO_0000118>
        <ns2:IAO_0000118 xml:lang="zh">A431 细胞</ns2:IAO_0000118>
        <ns2:CLO_0051635 xml:lang="zh">A431-物种鉴定-PUMC</ns2:CLO_0051635>
        <ns2:CLO_0051635 xml:lang="zh">A431-细胞形态-PUMC</ns2:CLO_0051635>
        <ns2:CLO_0037192 xml:lang="zh">Amelogenin：X；CSF1PO：11，12；D13S317：9，13；D16S539：12，14；D18S51：13，17；D19S433：15，15.2；D21S11：28；D2S1338：17，20；D3S1358：14，20；D5S818：12，13；D7S820：10；D8S1179：13；FGA：20；TH01：9；TPOX：11；vWA：15，17；</ns2:CLO_0037192>
        <ns2:CLO_0051633 xml:lang="zh">A类</ns2:CLO_0051633>
        <ns2:CLO_0051627 xml:lang="zh">DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS</ns2:CLO_0051627>
        <ns2:CLO_0051628 xml:lang="zh">P40</ns2:CLO_0051628>
        <ns2:IAO_0000119 xml:lang="zh">PMID: 8552591</ns2:IAO_0000119>
        <ns2:CLO_0051627 xml:lang="zh">http://www.cellresource.cn/pdf/20080108100710.pdf</ns2:CLO_0051627>
        <ns2:CLO_0051624 xml:lang="zh">上皮样</ns2:CLO_0051624>
        <ns2:CLO_0051636 xml:lang="zh">中国医学科学院基础医学研究所基础医学细胞中心</ns2:CLO_0051636>
        <ns2:CLO_0051623 xml:lang="zh">人皮肤鳞癌细胞；A-431 [A431]</ns2:CLO_0051623>
        <ns2:CLO_0051630 xml:lang="zh">培养法（-）</ns2:CLO_0051630>
        <ns2:CLO_0051629 xml:lang="zh">基础培养基+8%DMSO+20%FBS</ns2:CLO_0051629>
        <ns2:CLO_0051637 xml:lang="zh">杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com</ns2:CLO_0051637>
        <ns2:CLO_0051626 xml:lang="zh">该细胞源自一位患有皮肤鳞状细胞癌的85岁女性，是Giard DJ等人建立的一系列细胞株中的一株。该细胞在免疫抑制小鼠体内可成瘤，在琼脂上培养可形成克隆；是一个超三倍体人细胞株。</ns2:CLO_0051626>
        <ns2:CLO_0051625 xml:lang="zh">贴壁生长</ns2:CLO_0051625>
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        <foaf:depicted_by rdf:resource="http://www.cellresource.cn/pic/20090406125722.jpg"/>
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